THE ANALYSIS OF GENE EXPRESSION ON FERTILITY DECLINE IN CAENO-RHABDITIS ELEGANS AFTER THE TREATMENT WITH 5-FLUOROURACIL

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ZHANG SI; LU ZHAOLIAN; LIANG HONGMEI; FU XIMEI; YAN ZHANG; XIN LIU; BAO GENSHU; JING TAO; WANG XUAN; WANG MENG; WU JULONG; CHEN GEN

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Journal: Iranian Journal of Public Health Year:2015 | Volume:44 | Issue:8 Page(s): 1061-1071

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Title

THE ANALYSIS OF GENE EXPRESSION ON FERTILITY DECLINE IN CAENO-RHABDITIS ELEGANS AFTER THE TREATMENT WITH 5-FLUOROURACIL

Author(s)

Keywords

CAENORHABDITIS ELEGANS

5-FLUOROURACIL

DIGITAL GENE EXPRESSION

Abstract

Background: 5-FLUOROURACIL could lead to a decline in fertility in CAENORHABDITIS ELEGANS. The aim of this study was to describe the mechanisms underlying such an altered fertility phenotype and to illustrate the specific genes and path-ways that are involved in the related phenotypic changes in C. elegans.Methods: We isolated total RNA from the samples and used a new method called DIGITAL GENE EXPRESSION (DGE), which can rapidly identify genes with altered transcript levels. The random genes were confirmed by real-time RT-PCR.Results: We analyzed the results of two methods to draw conclusions based on a comparison between C. elegans and other harmful parasites. Compared with controls, 1147 genes were up-regulated, and 1067 were down-regulated. Overall, 101 up-regulated genes had a log2 ratio higher than 8, whereas the log2 ratio of 141 down-regulated genes was higher than 8. After mapping to the reference database, 4 pathways were confirmed to be involved in this phenomenon, with statistically significant participation from 19 genes.Conclusion: For the first time, the transcript sequence of 5-Fu-treated worms and controls was detected. We found that 4 possible pathways, i.e., ECM-receptor interaction pathway, TGF-beta signaling pathway, Focal adhesion and Hypertrophic cardiomyopathy, may be involved in the number decline in the embryos of C. elegans. Specifically, the ECM-receptor interaction pathway and Focal adhesion may be very important pathways that alter the reproduction of C. elegans.

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APA: Copy

ZHANG, SI, LU, ZHAOLIAN, LIANG, HONGMEI, FU, XIMEI, YAN, ZHANG, XIN, LIU, BAO, GENSHU, JING, TAO, WANG, XUAN, WANG, MENG, WU, JULONG, & CHEN, GEN. (2015). THE ANALYSIS OF GENE EXPRESSION ON FERTILITY DECLINE IN CAENO-RHABDITIS ELEGANS AFTER THE TREATMENT WITH 5-FLUOROURACIL. IRANIAN JOURNAL OF PUBLIC HEALTH, 44(8), 1061-1071. SID. https://sid.ir/paper/273873/en

Vancouver: Copy

ZHANG SI, LU ZHAOLIAN, LIANG HONGMEI, FU XIMEI, YAN ZHANG, XIN LIU, BAO GENSHU, JING TAO, WANG XUAN, WANG MENG, WU JULONG, CHEN GEN. THE ANALYSIS OF GENE EXPRESSION ON FERTILITY DECLINE IN CAENO-RHABDITIS ELEGANS AFTER THE TREATMENT WITH 5-FLUOROURACIL. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2015;44(8):1061-1071. Available from: https://sid.ir/paper/273873/en

IEEE: Copy

SI ZHANG, ZHAOLIAN LU, HONGMEI LIANG, XIMEI FU, ZHANG YAN, LIU XIN, GENSHU BAO, TAO JING, XUAN WANG, MENG WANG, JULONG WU, and GEN CHEN, “THE ANALYSIS OF GENE EXPRESSION ON FERTILITY DECLINE IN CAENO-RHABDITIS ELEGANS AFTER THE TREATMENT WITH 5-FLUOROURACIL,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 44, no. 8, pp. 1061–1071, 2015, [Online]. Available: https://sid.ir/paper/273873/en

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