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Information Journal Paper

Title

COMPARISON OF TWO DIFFERENT PCR-BASED METHODS FOR DETECTION OF GAA EXPANSIONS IN FRATAXIN GENE

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Abstract

 Expansion of GAA TRINUCLEOTIDE REPEATs is the molecular basis of FRIEDREICH’S ATAXIA (FRDA). Precise detection of the GAA expansion repeat in frataxin gene has always been a challenge. Different molecular methods have been suggested for detection of GAA expansion, including; short-PCR, LONG-PCR, TRIPLET REPEAT PRIMED-PCR (TP-PCR) and southern blotting. The aim of study was to evaluate two PCR-based methods, TP-PCR and LONG-PCR, and to explore the use of TP-PCR accompanying with LONG-PCR for accurate genotyping of FRDA patients.Methods: Blood samples were collected from six Iranian patients suspected to FRDA, who referred to the Depart-ment of Medical Genetics at Tehran University of Medical Sciences during the year 2014. For one of these patients’ four asymptomatic members of the family were also recruited for the analysis. DNA extraction was performed by two different methods. TP-PCR and LONG-PCR were carried out in all samples. The type of this study is assessment / in-vestigation of methods.Results: Using a combination of the above methods, the genotypes of all samples were confirmed as five homozyg-ous mutants (expanded GAA repeats), two heterozygous and three homozygous normal (normal repeat size). The results obtained by TP-PCR are consistent with LONG-PCR results.Conclusion: The presence or absence of expanded alleles can be identified correctly by TP-PCR. Performing LONG-PCR and Fluorescent-long-PCR enables accurate genotyping in all samples. This approach is highly reliable. It could be successfully used for detection of GAA expansion repeats.

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    APA: Copy

    ENTEZAM, MONA, AMIRFIROOZI, Akbar, TOGHA, MANSOUREH, & KERAMATIPOUR, MOHAMMAD. (2017). COMPARISON OF TWO DIFFERENT PCR-BASED METHODS FOR DETECTION OF GAA EXPANSIONS IN FRATAXIN GENE. IRANIAN JOURNAL OF PUBLIC HEALTH, 46(2), 0-0. SID. https://sid.ir/paper/274069/en

    Vancouver: Copy

    ENTEZAM MONA, AMIRFIROOZI Akbar, TOGHA MANSOUREH, KERAMATIPOUR MOHAMMAD. COMPARISON OF TWO DIFFERENT PCR-BASED METHODS FOR DETECTION OF GAA EXPANSIONS IN FRATAXIN GENE. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2017;46(2):0-0. Available from: https://sid.ir/paper/274069/en

    IEEE: Copy

    MONA ENTEZAM, Akbar AMIRFIROOZI, MANSOUREH TOGHA, and MOHAMMAD KERAMATIPOUR, “COMPARISON OF TWO DIFFERENT PCR-BASED METHODS FOR DETECTION OF GAA EXPANSIONS IN FRATAXIN GENE,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 46, no. 2, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/274069/en

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