QUANTIFICATION OF CDR1 GENE EXPRESSION IN FLUCONAZOLE RESISTANT CANDIDA GLABRATA STRAINS USING REAL-TIME PCR

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SHAHROKHI SHADI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Public Health Year:2017 | Volume:46 | Issue:8 Page(s): 1118-1122

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Title

QUANTIFICATION OF CDR1 GENE EXPRESSION IN FLUCONAZOLE RESISTANT CANDIDA GLABRATA STRAINS USING REAL-TIME PCR

Author(s)

SHAHROKHI SHADI | Issue Writer Certificate

Keywords

CANDIDA GLABRATA

CDR1

FLUCONAZOLE RESISTANCE

REAL-TIME PCR

Abstract

Background: The opportunistic fungi, particularly CANDIDA GLABRATA has been known as main etiologic agents of lifethreatinginfections in some patients. Although fluconazole is the most effective antifungal agent against candidiasis, C. glabrata, fluconazole-resistant strains have been increased recently overexpression or mutations of ATP-bindingcassette (ABC) transporter family membrane proteins such as; Cg CDR1, Cg CDR2 are responsible for fluconazoleresistance in a large proportion of candidiasis cases. The aim of this study was to evaluate CDR1 gene expression levelas one of main mechanism involved in this resistance using.Methods: CANDIDA GLABRATA strains were collected from various clinical samples in hospitals of Tehran in 2015. Aftervalidation of all isolates by conventional and molecular methods, the susceptibility analysis to fluconazole of all isolateswas performed using CLSI broth microdilution M27-A3 and M27-S4 protocols. Two isolates have been selected basedon difference in susceptibility and CDR1-mRNA expression level of isolates was measured by REAL-TIME PCR method.Results: Susceptibility results revealed that 32%, 64% and 4% of strains were susceptible, dose-dependent (DD) andresistant to fluconazole respectively. Furthermore, resistance strain of C. glabrata (MIC≥64 μg/ml) showed overexpressionof CDR1 compared with sensitive strain in REAL-TIME PCR analysis.Conclusion: Thus, it is necessary to investigate the functions of CgCDR1 genes as a transporter-related gene.

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APA: Copy

SHAHROKHI, SHADI. (2017). QUANTIFICATION OF CDR1 GENE EXPRESSION IN FLUCONAZOLE RESISTANT CANDIDA GLABRATA STRAINS USING REAL-TIME PCR. IRANIAN JOURNAL OF PUBLIC HEALTH, 46(8), 1118-1122. SID. https://sid.ir/paper/274224/en

Vancouver: Copy

SHAHROKHI SHADI. QUANTIFICATION OF CDR1 GENE EXPRESSION IN FLUCONAZOLE RESISTANT CANDIDA GLABRATA STRAINS USING REAL-TIME PCR. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2017;46(8):1118-1122. Available from: https://sid.ir/paper/274224/en

IEEE: Copy

SHADI SHAHROKHI, “QUANTIFICATION OF CDR1 GENE EXPRESSION IN FLUCONAZOLE RESISTANT CANDIDA GLABRATA STRAINS USING REAL-TIME PCR,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 46, no. 8, pp. 1118–1122, 2017, [Online]. Available: https://sid.ir/paper/274224/en

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