COLLAGEN EXTRACTED FROM PERSIAN GULF SQUID EXHIBITS ANTI-CYTOTOXIC PROPERTIES ON APPLE PECTIC TREATED CELLS: ASSESSMENT IN AN IN VITRO BIOASSAY MODEL

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DELPHI LADAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

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Journal: Iranian Journal of Public Health Year:2016 | Volume:45 | Issue:8 Page(s): 1054-1063

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Title

COLLAGEN EXTRACTED FROM PERSIAN GULF SQUID EXHIBITS ANTI-CYTOTOXIC PROPERTIES ON APPLE PECTIC TREATED CELLS: ASSESSMENT IN AN IN VITRO BIOASSAY MODEL

Author(s)

DELPHI LADAN | Issue Writer Certificate

Keywords

CELL CULTURE

COLLAGEN

PECTIC ACID

SQUID

3D CULTURE SYSTEM

Abstract

Background: COLLAGEN-based three-dimensional (3D) in vitro systems have been introduced to study the physiological states of cells. As a biomolecule, COLLAGEN is usually extracted from terrestrial animals whilst aquatic animals like SQUID contain large amounts of COLLAGEN.Methods: In order to make effective use of marine organisms, we selected Persian Gulf SQUID in 2015 to extract the required COLLAGEN. Then, a 3D CULTURE SYSTEM based on the extracted COLLAGEN was applied to investigate cellular mechanisms in a native microenvironment. The formed COLLAGEN gel was used to investigate the growth of MDA-MB-231 breast cancer cells as well as responses to PECTIC ACID.Results: The results revealed that the extracted COLLAGEN contained α, ß and γ components with high water holding capacity. This COLLAGEN formed a gel-like structure, which could promote the proliferation of MDA-MB-231 breast cancer cells. The MDA-MB-231 cells’ viability in presence of PECTIC ACID, demonstrating the cells’ behavior in a 3D CULTURE SYSTEM.Conclusion: It seems that the COLLAGEN extracted from SQUID skin has type I COLLAGEN properties. It might be used as a substrate in 3D CELL CULTURE systems.

Cites

Water Absorption of Chitosan, Collagen, and Chitosan/Collagen Blend Membranes Exposed to Gamma-Ray Irradiation

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APA: Copy

DELPHI, LADAN. (2016). COLLAGEN EXTRACTED FROM PERSIAN GULF SQUID EXHIBITS ANTI-CYTOTOXIC PROPERTIES ON APPLE PECTIC TREATED CELLS: ASSESSMENT IN AN IN VITRO BIOASSAY MODEL. IRANIAN JOURNAL OF PUBLIC HEALTH, 45(8), 1054-1063. SID. https://sid.ir/paper/274333/en

Vancouver: Copy

DELPHI LADAN. COLLAGEN EXTRACTED FROM PERSIAN GULF SQUID EXHIBITS ANTI-CYTOTOXIC PROPERTIES ON APPLE PECTIC TREATED CELLS: ASSESSMENT IN AN IN VITRO BIOASSAY MODEL. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2016;45(8):1054-1063. Available from: https://sid.ir/paper/274333/en

IEEE: Copy

LADAN DELPHI, “COLLAGEN EXTRACTED FROM PERSIAN GULF SQUID EXHIBITS ANTI-CYTOTOXIC PROPERTIES ON APPLE PECTIC TREATED CELLS: ASSESSMENT IN AN IN VITRO BIOASSAY MODEL,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 45, no. 8, pp. 1054–1063, 2016, [Online]. Available: https://sid.ir/paper/274333/en

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