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Information Journal Paper

Title

A NEW MONOCLONAL ANTIBODY RADIOPHARMACEUTICAL FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST CANCER: DIRECT LABELING OF ANTIBODY AND ITS QUALITY CONTROL

Pages

  51-56

Abstract

 Radioimmunoscintigraphy (RIS) has found widespread clinical application in tumor diagnosis. The antibody (Ab) PR81 is a new murine anti-MUC1 MONOCLONAL ANTIBODY (MAb) against human breast carcinoma. In this study a very simple, rapid and efficient method for labeling of this MAb with 99mTc, particularly suitable for development of a ‘kit’is described. The reduction of Ab was performed with 2-mercaptoethanol (2-ME) at a molar ratio of 2000:1 (2-ME:MAb) and the reduced Ab was labeled with 99mTc via methylene diphosphonate (MDP) as a transchelator. The labeling efficiency which was determined by instant thin layer chromatography (ITLC) was 94.2%±2.3. Radiocolloides measured by cellulose nitrate electrophoresis were 2.5%±1.7. In vitro stability of the labeled product in human serum which was measured by gel filtration chromatography (FPLC) was 70%±5.7 over 24 hr. The integrity of labeled MAb was checked by means of SDS-PAGE and no significant fragmentation was observed. The results of the cell-binding studies showed that both labeled and unlabeled PR81 were able to compete for binding to MCF 7 cells. Biodistribution studies were performed in normal BALB/c mice at 4 and 24 hrs post-injection and no important accumulation was observed in vital organs. These results show that the new radiopharmaceutical may be considered as a promising candidate for imaging of breast cancer.

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    Cite

    APA: Copy

    SALOUTI, M., RAJABI, H., BABAEI, M.H., NAJAFI, R., RASAEI, M.J., MOHAMMADNEZHAD, J., PAKNEZHAD, M., HASAN, M., & ALTARIHI, T.. (2006). A NEW MONOCLONAL ANTIBODY RADIOPHARMACEUTICAL FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST CANCER: DIRECT LABELING OF ANTIBODY AND ITS QUALITY CONTROL. DARU JOURNAL OF PHARMACEUTICAL SCIENCE, 14(1), 51-56. SID. https://sid.ir/paper/275325/en

    Vancouver: Copy

    SALOUTI M., RAJABI H., BABAEI M.H., NAJAFI R., RASAEI M.J., MOHAMMADNEZHAD J., PAKNEZHAD M., HASAN M., ALTARIHI T.. A NEW MONOCLONAL ANTIBODY RADIOPHARMACEUTICAL FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST CANCER: DIRECT LABELING OF ANTIBODY AND ITS QUALITY CONTROL. DARU JOURNAL OF PHARMACEUTICAL SCIENCE[Internet]. 2006;14(1):51-56. Available from: https://sid.ir/paper/275325/en

    IEEE: Copy

    M. SALOUTI, H. RAJABI, M.H. BABAEI, R. NAJAFI, M.J. RASAEI, J. MOHAMMADNEZHAD, M. PAKNEZHAD, M. HASAN, and T. ALTARIHI, “A NEW MONOCLONAL ANTIBODY RADIOPHARMACEUTICAL FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST CANCER: DIRECT LABELING OF ANTIBODY AND ITS QUALITY CONTROL,” DARU JOURNAL OF PHARMACEUTICAL SCIENCE, vol. 14, no. 1, pp. 51–56, 2006, [Online]. Available: https://sid.ir/paper/275325/en

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