IDENTIFICATION AND AMPLIFICATION OF HYOSCYAMINE 6B- HYDROXYLASE GENE IN GENOMIC DNAOF IN VITRO CULTURED ROOTS OF HYOSCYAMUS NIGER L. BY POLYMRASE CHAIN
REACTION

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EBRAHIMZADEH H.; TEYMOURI A.; LOHRASEBI T.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DARU Journal of Pharmaceutical Sciences Year:2000 | Volume:8 | Issue:1-2 Page(s): 36-41

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Information Journal Paper

Title

IDENTIFICATION AND AMPLIFICATION OF HYOSCYAMINE 6B- HYDROXYLASE GENE IN GENOMIC DNAOF IN VITRO CULTURED ROOTS OF HYOSCYAMUS NIGER L. BY POLYMRASE CHAIN REACTION

Author(s)

EBRAHIMZADEH H. | TEYMOURI A. | LOHRASEBI T. | Issue Writer Certificate

Keywords

HYOSCYAMUS NIGER

CULTURED ROOTS

H6H GENE

Abstract

The H6H GENE for hyoscyamine 6β-hydroxylase (H6H), which converts hyoscyamine to scopolamine, was isolated from HYOSCYAMUS NIGER. The roots of 14 days sterile seedlings were transferred to a modified liquid Bs medium containing 1 mM indolebutyric acid, and after appearance of the lateral roots, subcultured in a free hormone medium. Following a week, its genomic DNA was extracted and PCR performed. By extraction an accurate DNA and using suitable primers, H6H GENE can be isolated completely.

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