EFFECT OF PARAOXON ON GABA UPTAKE BY RAT CEREBRAL CORTEX SYNAPTOSOMES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHASEMI A.; SADIDI A.; ASGARI A.; KHOUSHBATEN A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS) Year:2006 | Volume:31 | Issue:3 Page(s): 125-130

Download Full-Text

View:

268

Download:

Cites:

Information Journal Paper

Title

EFFECT OF PARAOXON ON GABA UPTAKE BY RAT CEREBRAL CORTEX SYNAPTOSOMES

Author(s)

GHASEMI A. | SADIDI A. | ASGARI A. | KHOUSHBATEN A. | Issue Writer Certificate

Keywords

SYNAPTOSOME

GABA

ORGANOPHOSPHATE

PARAOXON

Abstract

Background: It has been suggested that ORGANOPHOSPHATEs may inhibit gamma- aminobutyric acid (GABA) metabolism in synaptosomal preparations. In the present investigation, we have assessed the interaction between PARAOXON and the GABA system at synaptic level. Methods: SYNAPTOSOMEs were prepared from male Wistar rats (200-250 g). Cerebral cortex was dissected and homogenized, then centrifuged at 1000g for 5 min and again at 12000g for 20 min. The pellet containing SYNAPTOSOMEs was resuspended in the buffer solution and the protein concentration adjusted at 1 mg/ml. Determination of lactate dehydrogenase (LDH) activity, as a biochemical index for synaptosomal integrity, was assessed. Cholinesterase activity of SYNAPTOSOMEs was also determined. SYNAPTOSOMEs were preincubated for 20 min with two concentrations of PARAOXON (10-9-10-3M), and then incubated with [3H] GABA for 10 min, before being washed through 0.65 mm filters. Results: PARAOXON inhibited cholinesterase activity of SYNAPTOSOMEs in a concentration dependent manner. Synaptosomal accumulation of [3H] GABA/GABA was time dependent and peaked at 15 min. PARAOXON significantly increased the uptake in nano molar concentrations and decreased it at higher concentrations. Conclusion: The result of this study indicate that in SYNAPTOSOMEs prepared from rat cerebral cortex PARAOXON increases GABA uptake at low dose and inhibits its uptake at high doses. This may imply a role for ORGANOPHOSPHATE-induced convulsion, which needs further clarification.

Cites

No record.

References

No record.

Cite

APA: Copy

GHASEMI, A., SADIDI, A., ASGARI, A., & KHOUSHBATEN, A.. (2006). EFFECT OF PARAOXON ON GABA UPTAKE BY RAT CEREBRAL CORTEX SYNAPTOSOMES. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 31(3), 125-130. SID. https://sid.ir/paper/276046/en

Vancouver: Copy

GHASEMI A., SADIDI A., ASGARI A., KHOUSHBATEN A.. EFFECT OF PARAOXON ON GABA UPTAKE BY RAT CEREBRAL CORTEX SYNAPTOSOMES. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2006;31(3):125-130. Available from: https://sid.ir/paper/276046/en

IEEE: Copy

A. GHASEMI, A. SADIDI, A. ASGARI, and A. KHOUSHBATEN, “EFFECT OF PARAOXON ON GABA UPTAKE BY RAT CEREBRAL CORTEX SYNAPTOSOMES,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 31, no. 3, pp. 125–130, 2006, [Online]. Available: https://sid.ir/paper/276046/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops