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Information Journal Paper

Title

INHIBITION OF IL-13 BY ANTISENSE OLIGONUCLEOTIDE CHANGES IMMUNOGLOBULIN ISOTYPE PROFILE IN CULTURED B-LYMPHOCYTES

Pages

  185-191

Keywords

IGE 

Abstract

 The link between IL-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. At the present study, we address the role of B cell derived IL-13 in the IGE and other immunoglobulin development. ANTISENSE OLIGO for human IL-13 m-RNA was used to study IGE down regulation. Human B-lymphocytes were purified by positive selection using magnetic cell sorting and were cultured in the complete medium plus anti-CD40 monoclonal antibody and recombinant human IL-4. Immunoglobulin assay was performed by ELISA in the presence and absence of ANTISENSE OLIGOnucleotide. We demonstrated that IL-13 antisense causes the decrease of IGE and increase of IgA significantly and no significant changes in IgM and IgG levels (p<0.01). We also demonstrated that both IL-13 inhibition and IL-4 removal cause the complete blocking of IGE and significant decrease of IgM and IgG levels. Our IL-13 ANTISENSE OLIGO can block B-cell IL-13 productions and consequently inhibits IGE production followed by IgA class switching in vitro. We suggest that in contrast to the IL-4, IL-13 is apparently more potent in the IGE switching and has no significant role in IgG and IgM levels.

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  • Cite

    APA: Copy

    MOUSAVI, T., MAZER, B., & TEBIANIAN, M.. (2004). INHIBITION OF IL-13 BY ANTISENSE OLIGONUCLEOTIDE CHANGES IMMUNOGLOBULIN ISOTYPE PROFILE IN CULTURED B-LYMPHOCYTES . IRANIAN BIOMEDICAL JOURNAL, 8(4), 185-191. SID. https://sid.ir/paper/276982/en

    Vancouver: Copy

    MOUSAVI T., MAZER B., TEBIANIAN M.. INHIBITION OF IL-13 BY ANTISENSE OLIGONUCLEOTIDE CHANGES IMMUNOGLOBULIN ISOTYPE PROFILE IN CULTURED B-LYMPHOCYTES . IRANIAN BIOMEDICAL JOURNAL[Internet]. 2004;8(4):185-191. Available from: https://sid.ir/paper/276982/en

    IEEE: Copy

    T. MOUSAVI, B. MAZER, and M. TEBIANIAN, “INHIBITION OF IL-13 BY ANTISENSE OLIGONUCLEOTIDE CHANGES IMMUNOGLOBULIN ISOTYPE PROFILE IN CULTURED B-LYMPHOCYTES ,” IRANIAN BIOMEDICAL JOURNAL, vol. 8, no. 4, pp. 185–191, 2004, [Online]. Available: https://sid.ir/paper/276982/en

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