THE DEMONSTRATION OF POLYETHYLENIMINE MEDIATED GENE TRANSFER INTO THE RODENT HYPOTHALAMUS RESULTS IN PERSISTENT OVER-EXPRESSION AND PHENOTYPIC CHANGE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAHMOUDI M.; GARDINER J.V.; LOTFI A.S.; BLOOM S.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2004 | Volume:8 | Issue:1 Page(s): 33-39

Download Full-Text

View:

417

Download:

129

Cites:

Information Journal Paper

Title

THE DEMONSTRATION OF POLYETHYLENIMINE MEDIATED GENE TRANSFER INTO THE RODENT HYPOTHALAMUS RESULTS IN PERSISTENT OVER-EXPRESSION AND PHENOTYPIC CHANGE

Author(s)

MAHMOUDI M. | GARDINER J.V. | LOTFI A.S. | BLOOM S.R. | Issue Writer Certificate

Keywords

NEUROPEPTIDE Y (NPY)

GENE DELIVERY

POLYETHYLENIMINE (PEI)

RAT

Abstract

Polyethylenimine (PEI) has been proposed as a non-viral vector, and has been successfully used to transfer reporter genes into the central nervous system (CNS), kidneys, and lungs of adult mice. Neuropeptide Y (NPY) is a peptide expressed in the hypothalamus and is important in the regulation of body weight. Using PEI combined with stereotactic microinjection, we have successfully transferred cDNA-encoding NPY driven by the cytomegalovirus (CMV) promoter into the arcuate nucleus of adult male Wistar RATs. Animals treated with NPY expressing plasmids (pNPY) gained more weight than the controls (p<0.05), with associated increases in food intake (p<0.05) and decreased brown adipose tissue activity, measured by Guanosine Diphosphate (GDP) binding to mitochondria, (p<0.05). In a separate study, hypothalamic slices from the RATs treated with pNPY/PEI showed increased NPY release (pNPY 9.7 ± 0.3 fmol/l vs. control 8.3 ± 0.5 fmol/l, p<0.05, n = 3). These results suggest that PEI is an effective vector for gene transfer into the rodent brain and can increase the protein production sufficient to result a persistent phenotypic change. This technique offers the potential of a simple and effective method to manipulate gene expression localised to specific regions of the adult rodent brain.

Cites

No record.

References

No record.

Cite

APA: Copy

MAHMOUDI, M., GARDINER, J.V., LOTFI, A.S., & BLOOM, S.R.. (2004). THE DEMONSTRATION OF POLYETHYLENIMINE MEDIATED GENE TRANSFER INTO THE RODENT HYPOTHALAMUS RESULTS IN PERSISTENT OVER-EXPRESSION AND PHENOTYPIC CHANGE . IRANIAN BIOMEDICAL JOURNAL, 8(1), 33-39. SID. https://sid.ir/paper/277004/en

Vancouver: Copy

MAHMOUDI M., GARDINER J.V., LOTFI A.S., BLOOM S.R.. THE DEMONSTRATION OF POLYETHYLENIMINE MEDIATED GENE TRANSFER INTO THE RODENT HYPOTHALAMUS RESULTS IN PERSISTENT OVER-EXPRESSION AND PHENOTYPIC CHANGE . IRANIAN BIOMEDICAL JOURNAL[Internet]. 2004;8(1):33-39. Available from: https://sid.ir/paper/277004/en

IEEE: Copy

M. MAHMOUDI, J.V. GARDINER, A.S. LOTFI, and S.R. BLOOM, “THE DEMONSTRATION OF POLYETHYLENIMINE MEDIATED GENE TRANSFER INTO THE RODENT HYPOTHALAMUS RESULTS IN PERSISTENT OVER-EXPRESSION AND PHENOTYPIC CHANGE ,” IRANIAN BIOMEDICAL JOURNAL, vol. 8, no. 1, pp. 33–39, 2004, [Online]. Available: https://sid.ir/paper/277004/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops