EXTRACTION OF THE OUTER MEMBRANE PROTEINS OF H. PYLORI AND EVALUATION OF THEIR PRESENCE IN STOOL OF THE INFECTED INDIVIDUALS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHEYKHIAN A.; ZAHIR MOHAMMAD HASSAN; SHOKRI F.; MALEKZADEH R.; SIAVASHI F.; MUSTAFAIE A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2004 | Volume:8 | Issue:2 Page(s): 83-88

Download Full-Text

View:

439

Download:

197

Cites:

Information Journal Paper

Title

EXTRACTION OF THE OUTER MEMBRANE PROTEINS OF H. PYLORI AND EVALUATION OF THEIR PRESENCE IN STOOL OF THE INFECTED INDIVIDUALS

Author(s)

Keywords

H. PYLORI

OUTER MEMBRANE PROTEINS (OMP)

STOOL

SDS-PAGE

AFFINITY CHROMATOGRAPHY

Abstract

Helicobacter pylori infections are accompanied with the release of some antigenic material into the STOOL. Outer membranes proteins (OMP) are among the major antigens of bacteria, which in comparison with other bacterial constituents have less cross-reactivity. In this study, OMP of H. PYLORI were isolated from 11 clinical isolates and rabbit antiserum against them was used to detect possible antigens of H. PYLORI, which are released into the STOOL. We used immunoblotting and AFFINITY CHROMATOGRAPHY to detect such antigens in fecal antigenic extracts of infected individuals. By immunoblotting, we were able to detect a 26 kDa band under reducing and non-reducing conditions, but many more antigens (at least 5 antigens with molecular weights of about 14, 26, 52, 57.5 and 66 kDa) were isolated by AFFINITY CHROMATOGRAPHY. The 26 kDa antigen had a higher concentration and is seen in nearly all positive samples. Since the 26 kDa antigen is detectable by these two techniques, we suggest that this antigen is one of the major antigens of H. PYLORI which is released into the STOOL and can be considered as a candidate diagnostic antigen to be used in diagnostic kit development.

Cites

PREPARATIVE SDS-PAGE ELECTROELUTION FOR RAPID PURIFICATION OF ALKYL HYDROPEROXIDE REDUCTASE FROM HELICOBACTER PYLORI (SHORT COMMUNICATION)

References

No record.

Cite

APA: Copy

SHEYKHIAN, A., ZAHIR, MOHAMMAD HASSAN, SHOKRI, F., MALEKZADEH, R., SIAVASHI, F., & MUSTAFAIE, A.. (2004). EXTRACTION OF THE OUTER MEMBRANE PROTEINS OF H. PYLORI AND EVALUATION OF THEIR PRESENCE IN STOOL OF THE INFECTED INDIVIDUALS. IRANIAN BIOMEDICAL JOURNAL, 8(2), 83-88. SID. https://sid.ir/paper/277011/en

Vancouver: Copy

SHEYKHIAN A., ZAHIR MOHAMMAD HASSAN, SHOKRI F., MALEKZADEH R., SIAVASHI F., MUSTAFAIE A.. EXTRACTION OF THE OUTER MEMBRANE PROTEINS OF H. PYLORI AND EVALUATION OF THEIR PRESENCE IN STOOL OF THE INFECTED INDIVIDUALS. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2004;8(2):83-88. Available from: https://sid.ir/paper/277011/en

IEEE: Copy

A. SHEYKHIAN, MOHAMMAD HASSAN ZAHIR, F. SHOKRI, R. MALEKZADEH, F. SIAVASHI, and A. MUSTAFAIE, “EXTRACTION OF THE OUTER MEMBRANE PROTEINS OF H. PYLORI AND EVALUATION OF THEIR PRESENCE IN STOOL OF THE INFECTED INDIVIDUALS,” IRANIAN BIOMEDICAL JOURNAL, vol. 8, no. 2, pp. 83–88, 2004, [Online]. Available: https://sid.ir/paper/277011/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops