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Information Journal Paper

Title

THE EFFECT OF HEAT SHOCK ON PRODUCTION OF RECOMBINANT HUMAN INTERFERON ALPHA 2A (RHIFN -2A) BY ESCHERICHIA COLI

Pages

  155-162

Keywords

RECOMBINANT HUMAN INTERFERON ALPHA 2A (RHIFN ل-2A) 
HEAT SHOCK PROTEIN (HSP) 

Abstract

 Recombinant human interferon alpha 2a (rhIFN T-2a) production and cell growth were monitored in a set of genetically modified E. coli strains (MSD1519, MSD1520, MSD 1521, MSD 1522, MSD 1523) producing rhIFN T-2a. The growth was followed at OD 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (PyMS) of cell biomass and RECOMBINANT PROTEIN production was determined by SDS-gel electrophoresis. The HEAT STRESS applied was minimal (50°C for 5 minutes) but the effects were detected in most of the strains. All the strains except MSD 1520 showed a significant increase in the quantity of the rhIFN T-2a secretion at 25 hour growth under the heat shock condition, quantitated by the Bio-Rad Molecular analyst software, MultiAnalyst from the digital image of gels captured using a Fluor S image analysis system. In the main fermentation system at T7 hour, only MSD 1523 showed an increase in the rhIFN T-2a secretion under the heat shock condition, at T8 hour MSD 1520 and MSD 1523 had an increased rhIFN T-2a secretion, and at T10 MSD 1521 and MSD 1522 had an increased rhIFN T- 2a secretion under the heat shock condition. The PCCV ordination diagrams obtained from the PyMS result showed, a considerable effect of heat shock on the MSD 1519 strain at T5 hour. For the other strains, the result largely agreed with both the growth curves and the rhIFN T-2a production that had a limited effect on E. coli cultures. The increase of temperature in the main fermentation during the log phase of the bacterial culture during rhIFN T-2a expression depends on the strain specificity. This situation could definitely lead to over expression of the gene and higher intracellular accumulation of rhIFN T-2a molecule.

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    APA: Copy

    ROY, R.K., SAPATNEKAR, SH.M., & DESHMUKH, R.A.. (2005). THE EFFECT OF HEAT SHOCK ON PRODUCTION OF RECOMBINANT HUMAN INTERFERON ALPHA 2A (RHIFN -2A) BY ESCHERICHIA COLI. IRANIAN BIOMEDICAL JOURNAL, 9(4), 155-162. SID. https://sid.ir/paper/277097/en

    Vancouver: Copy

    ROY R.K., SAPATNEKAR SH.M., DESHMUKH R.A.. THE EFFECT OF HEAT SHOCK ON PRODUCTION OF RECOMBINANT HUMAN INTERFERON ALPHA 2A (RHIFN -2A) BY ESCHERICHIA COLI. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2005;9(4):155-162. Available from: https://sid.ir/paper/277097/en

    IEEE: Copy

    R.K. ROY, SH.M. SAPATNEKAR, and R.A. DESHMUKH, “THE EFFECT OF HEAT SHOCK ON PRODUCTION OF RECOMBINANT HUMAN INTERFERON ALPHA 2A (RHIFN -2A) BY ESCHERICHIA COLI,” IRANIAN BIOMEDICAL JOURNAL, vol. 9, no. 4, pp. 155–162, 2005, [Online]. Available: https://sid.ir/paper/277097/en

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