COMPARISON OF EMBRYO DEVELOPMENT BETWEEN INTRACYTOPLASMIC AND PIEZO-ASSISTED SPERM INJECTION AFTER TREATING MOUSE SPERMS BY CA2+ IONOPHORE

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SHAHVERDI A.; MOVAHEDIN M.; REZAZADEH VALOUJERDI M.; KAZEMI ASHTIANI S.

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Journal: Iranian Biomedical Journal Year:2007 | Volume:11 | Issue:4 Page(s): 245-250

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Title

COMPARISON OF EMBRYO DEVELOPMENT BETWEEN INTRACYTOPLASMIC AND PIEZO-ASSISTED SPERM INJECTION AFTER TREATING MOUSE SPERMS BY CA2+ IONOPHORE

Author(s)

SHAHVERDI A. | MOVAHEDIN M. | REZAZADEH VALOUJERDI M. | KAZEMI ASHTIANI S. | Issue Writer Certificate

Keywords

INTRACYTOPLASMIC SPERM INJECTION (ICSI)

PIEZO

SPERM ACTIVATION

EMBRYO DEVELOPMENT

Abstract

Background: The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and PIEZO-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse EMBRYO DEVELOPMENT. Methods: In this study, the conventional ICSI and PIEZO-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without CaI treated sperms. Results: PIEZO-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, P<0.001) and a cleavage rate of 73% (conventional ICSI: 58%, P<0.05). When the PIEZO-ICSI procedure was performed with CaI-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, P<0.05). However, the fertilization rate did not change significantly (64% vs. 56%). Conclusion: The PIEZO-ICSI accompanies with CaI-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos.

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APA: Copy

SHAHVERDI, A., MOVAHEDIN, M., REZAZADEH VALOUJERDI, M., & KAZEMI ASHTIANI, S.. (2007). COMPARISON OF EMBRYO DEVELOPMENT BETWEEN INTRACYTOPLASMIC AND PIEZO-ASSISTED SPERM INJECTION AFTER TREATING MOUSE SPERMS BY CA²⁺ IONOPHORE. IRANIAN BIOMEDICAL JOURNAL, 11(4), 245-250. SID. https://sid.ir/paper/277157/en

Vancouver: Copy

SHAHVERDI A., MOVAHEDIN M., REZAZADEH VALOUJERDI M., KAZEMI ASHTIANI S.. COMPARISON OF EMBRYO DEVELOPMENT BETWEEN INTRACYTOPLASMIC AND PIEZO-ASSISTED SPERM INJECTION AFTER TREATING MOUSE SPERMS BY CA²⁺ IONOPHORE. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2007;11(4):245-250. Available from: https://sid.ir/paper/277157/en

IEEE: Copy

A. SHAHVERDI, M. MOVAHEDIN, M. REZAZADEH VALOUJERDI, and S. KAZEMI ASHTIANI, “COMPARISON OF EMBRYO DEVELOPMENT BETWEEN INTRACYTOPLASMIC AND PIEZO-ASSISTED SPERM INJECTION AFTER TREATING MOUSE SPERMS BY CA²⁺ IONOPHORE,” IRANIAN BIOMEDICAL JOURNAL, vol. 11, no. 4, pp. 245–250, 2007, [Online]. Available: https://sid.ir/paper/277157/en

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