IMMUNOCYTOCHEMICAL STUDY ON MICROTUBULE REORGANIZATION IN HL-60 LEUKEMIA CELLS UNDERGOING APOPTOSIS

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AHMADIAN SH.; PAZHANG Y.; SHARIF TABRIZI A.

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Journal: Iranian Biomedical Journal Year:2007 | Volume:11 | Issue:4 Page(s): 209-214

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Title

IMMUNOCYTOCHEMICAL STUDY ON MICROTUBULE REORGANIZATION IN HL-60 LEUKEMIA CELLS UNDERGOING APOPTOSIS

Author(s)

AHMADIAN SH. | PAZHANG Y. | SHARIF TABRIZI A. | Issue Writer Certificate

Keywords

APOPTOSIS

MICROTUBULE

IMMUNOCYTOCHEMISTRY

Abstract

Background: MICROTUBULEs (MT) are important components of cell cytoskeleton and play key roles in cell motility mitosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing APOPTOSIS induced by cytotoxic drugs. The aim of this study was to correlate the changes in the MT with behavior of the g-tubulin in apoptotic cell, and to see if apoptitic MT showed biochemical characteristics of stable MT.Methods: APOPTOSIS was induced in the human leukemia cells (HL-60) by treatment with 1 mM of all-trans retinoic acid over a 5-day period. The time course of changes was assessed using flow cytometry, DNA fragmentation and IMMUNOCYTOCHEMISTRY in cells labeled for a-tubulins, acetylated a-tubulin and g-tubulin.Results: The results indicated that γ-tubulin content is increased after cells have gone through the APOPTOSIS with a diffuse cytoplasmic pattern. a-tubulin did not reveal any specific pattern of polymerization in apoptotic cells and acetylated a-tubulin content was also decreased in comparison with non-apoptotic cells.Conclusion: Our results support the idea that MICROTUBULE reorganization is an important factor of the mammalian cells response to APOPTOSIS, and the altered properties of the MT did not reflect changes in function as APOPTOSIS progresses

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APA: Copy

AHMADIAN, SH., PAZHANG, Y., & SHARIF TABRIZI, A.. (2007). IMMUNOCYTOCHEMICAL STUDY ON MICROTUBULE REORGANIZATION IN HL-60 LEUKEMIA CELLS UNDERGOING APOPTOSIS. IRANIAN BIOMEDICAL JOURNAL, 11(4), 209-214. SID. https://sid.ir/paper/277158/en

Vancouver: Copy

AHMADIAN SH., PAZHANG Y., SHARIF TABRIZI A.. IMMUNOCYTOCHEMICAL STUDY ON MICROTUBULE REORGANIZATION IN HL-60 LEUKEMIA CELLS UNDERGOING APOPTOSIS. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2007;11(4):209-214. Available from: https://sid.ir/paper/277158/en

IEEE: Copy

SH. AHMADIAN, Y. PAZHANG, and A. SHARIF TABRIZI, “IMMUNOCYTOCHEMICAL STUDY ON MICROTUBULE REORGANIZATION IN HL-60 LEUKEMIA CELLS UNDERGOING APOPTOSIS,” IRANIAN BIOMEDICAL JOURNAL, vol. 11, no. 4, pp. 209–214, 2007, [Online]. Available: https://sid.ir/paper/277158/en

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