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Information Journal Paper

Title

MOLECULAR CHARACTERIZATION OF A SQUALENE EPOXIDASE GENE IN DERMATOPHYTE PATHOGEN TRICHOPHYTON TONSURANS

Pages

  55-58

Abstract

 Background: TRICHOPHYTON TONSURANS is one of the DERMATOPHYTE fungi which invades the skin and hair of human. Several properties of this fungus have been investigated so far. However a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the SQUALENE EPOXIDASE gene which is related to synthesis of ERGOSTEROL in this fungus. Methods: Pairs of 23 and 24 nucleotides primers were designed from highly conserved regions of the similar genes in other fungi. Mentioned primers were utilized in PCR by using isolated genomic DNA of T. tonsurans whereas the PCR fragments were then sequenced. Results and Conclusion: Nucleotides (n = 558) have been sequenced from this new gene which encodes a polypeptide with 186 amino acids. Sequences comparison in gene data banks (NCBI, NIH) for this part of DNA and its deduced amino acid revealed ignificant homology with members of the eukaryotic SQUALENE EPOXIDASE.

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    APA: Copy

    MOTEVAZE, K., NAMVAR, Z., EMAMI, M., NOURBAKHSH, FATEMEH, & REZAEI, SASAN. (2008). MOLECULAR CHARACTERIZATION OF A SQUALENE EPOXIDASE GENE IN DERMATOPHYTE PATHOGEN TRICHOPHYTON TONSURANS . IRANIAN BIOMEDICAL JOURNAL, 12(1), 55-58. SID. https://sid.ir/paper/277173/en

    Vancouver: Copy

    MOTEVAZE K., NAMVAR Z., EMAMI M., NOURBAKHSH FATEMEH, REZAEI SASAN. MOLECULAR CHARACTERIZATION OF A SQUALENE EPOXIDASE GENE IN DERMATOPHYTE PATHOGEN TRICHOPHYTON TONSURANS . IRANIAN BIOMEDICAL JOURNAL[Internet]. 2008;12(1):55-58. Available from: https://sid.ir/paper/277173/en

    IEEE: Copy

    K. MOTEVAZE, Z. NAMVAR, M. EMAMI, FATEMEH NOURBAKHSH, and SASAN REZAEI, “MOLECULAR CHARACTERIZATION OF A SQUALENE EPOXIDASE GENE IN DERMATOPHYTE PATHOGEN TRICHOPHYTON TONSURANS ,” IRANIAN BIOMEDICAL JOURNAL, vol. 12, no. 1, pp. 55–58, 2008, [Online]. Available: https://sid.ir/paper/277173/en

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