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Cites:

1

Information Journal Paper

Title

REPAIR OF SPINAL CORD INJURY BY CO-TRANSPLANTATION OF EMBRYONIC STEM CELL-DERIVED MOTOR NEURON AND OLFACTORY ENSHEATHING CELL

Pages

  125-135

Keywords

SPINAL CORD INJURY (SCI) 
EMBRYONIC STEM (ES) CELL 
OLFACTORY ENSHEATHING CELL (OEC) 

Abstract

 Background: The failure of regeneration after spinal cord injury (SCI) has been attributed to axonal demyelination and neuronal death. Cellular replacement and white matter regeneration are both necessary for SCI repair. In this study, we evaluated the co-transplantation of olfactory ensheathing cells (OEC) and embryonic stem (ES) cell-derived MOTOR NEURONs (ESMN) on contused SCI. Methods: OEC cultured from olfactory nerve rootlets and olfactory bulbs. ESMN was generated by exposing mouse ES cells to retinoic acid and sonic hedgehog. Thirty female rats were used to prepare SCI models in five groups. Control and medium injected groups was subjected to induce lesion without cell transplantation. OEC or ESMN or both were transplanted into the site of the lesion in other groups. Results: The purity of OEC culture was 95%. MOTOR NEURON progenitor markers (Olig2, Nkx6.1 and Pax6) and MOTOR NEURON markers (Isl1, Isl2 and Hb9) were expressed. Histological analysis showed that significantly more (P<0.001) spinal tissue was spared in OEC, ESMN and OEC+ ESMN groups but the OEC+ ESMN group had a significantly greater percentage of spared tissue and myelination than other groups (P< 0.05). The numbers of ESMN in co-transplanted group were significantly higher than ESMN group (P<0.05). A significant (P<0.05) recovery of hindlimb function was observed in rats in the transplanted groups. Conclusion: We found that the co-transplantation of ESMN and OEC into an injured spinal cord has a synergistic effect, promoting neural regeneration, ESMN survival and partial functional recovery.

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  • Cite

    APA: Copy

    SALEHI, MOHAMMAD, PASBAKHSH, P., SOLEYMANI, MASOUD, ABBASI, MAHDI, HASANZADEH, GH.R., MODARESI, M.H., & SOBHANI, A.GH.. (2009). REPAIR OF SPINAL CORD INJURY BY CO-TRANSPLANTATION OF EMBRYONIC STEM CELL-DERIVED MOTOR NEURON AND OLFACTORY ENSHEATHING CELL. IRANIAN BIOMEDICAL JOURNAL, 13(3), 125-135. SID. https://sid.ir/paper/277228/en

    Vancouver: Copy

    SALEHI MOHAMMAD, PASBAKHSH P., SOLEYMANI MASOUD, ABBASI MAHDI, HASANZADEH GH.R., MODARESI M.H., SOBHANI A.GH.. REPAIR OF SPINAL CORD INJURY BY CO-TRANSPLANTATION OF EMBRYONIC STEM CELL-DERIVED MOTOR NEURON AND OLFACTORY ENSHEATHING CELL. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2009;13(3):125-135. Available from: https://sid.ir/paper/277228/en

    IEEE: Copy

    MOHAMMAD SALEHI, P. PASBAKHSH, MASOUD SOLEYMANI, MAHDI ABBASI, GH.R. HASANZADEH, M.H. MODARESI, and A.GH. SOBHANI, “REPAIR OF SPINAL CORD INJURY BY CO-TRANSPLANTATION OF EMBRYONIC STEM CELL-DERIVED MOTOR NEURON AND OLFACTORY ENSHEATHING CELL,” IRANIAN BIOMEDICAL JOURNAL, vol. 13, no. 3, pp. 125–135, 2009, [Online]. Available: https://sid.ir/paper/277228/en

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