PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES RECOGNIZING A COMMON 57-KDA ANTIGEN OFLEISHMANIA SPECIES

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NEJAD MOGHADDAM AMIR; ABOLHASSANI MOHSEN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian Biomedical Journal Year:2009 | Volume:13 | Issue:4 Page(s): 245-251

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Title

PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES RECOGNIZING A COMMON 57-KDA ANTIGEN OFLEISHMANIA SPECIES

Author(s)

NEJAD MOGHADDAM AMIR | ABOLHASSANI MOHSEN | Issue Writer Certificate

Keywords

MONOCLONAL ANTIBODY (MAB)

LEISHMANIA

57-KDA PROTEIN

Abstract

Background: The therapy of LEISHMANIA infection is difficult and each year 1.5 million new cases of cutaneous LEISHMANIAsis and 500, 000 new cases of visceral LEISHMANIAsis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of LEISHMANIA antigens. In this report, we produced several mAb againstleishmania infantum antigens for antigen purification to be used as candidate vaccine.Methods: BALB/c mice were injected with freeze-thawed promastigote twice together with Freund adjuvant. Three days before fusion, antigen in saline was injected into the tail vain and then mice were killed and the spleen lymphocytes were fused with myeloma SP2/0.Results: Five mAb against promastigote form of LEISHMANIA infantum parasite were obtained.Western-blot analysis showed that these mAb recognize a band of 57- kDa protein either in parasite lysate or on wholeL. infantum, L. tropica, L. major and L. donovani. It seems that the 57 kDa-protein is the major surface LEISHMANIA antigen (gp63) that is neither stage-specific nor differentially regulated. These mAb do not recognize the recombinant gp63 antigen and seems recognizing only the native form of a gp63 isoform. The IgG1 mAb was purified by affinity column and was used to purify 57 kDa antigens from LEISHMANIA lysate.Conclusion: Since these antibodies recognizing one specific protein band in 4 different strains of LEISHMANIA, they could be used for LEISHMANIA diagnostic kits and also for purification of antigen to be tested for its protective effect against LEISHMANIA infection.

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APA: Copy

NEJAD MOGHADDAM, AMIR, & ABOLHASSANI, MOHSEN. (2009). PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES RECOGNIZING A COMMON 57-KDA ANTIGEN OFLEISHMANIA SPECIES. IRANIAN BIOMEDICAL JOURNAL, 13(4), 245-251. SID. https://sid.ir/paper/277242/en

Vancouver: Copy

NEJAD MOGHADDAM AMIR, ABOLHASSANI MOHSEN. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES RECOGNIZING A COMMON 57-KDA ANTIGEN OFLEISHMANIA SPECIES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2009;13(4):245-251. Available from: https://sid.ir/paper/277242/en

IEEE: Copy

AMIR NEJAD MOGHADDAM, and MOHSEN ABOLHASSANI, “PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES RECOGNIZING A COMMON 57-KDA ANTIGEN OFLEISHMANIA SPECIES,” IRANIAN BIOMEDICAL JOURNAL, vol. 13, no. 4, pp. 245–251, 2009, [Online]. Available: https://sid.ir/paper/277242/en

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