EFFECT OF CHRONIC INTRACEREBROVENTRICLUAR ADMINISTRATION OF LIPOPOLYSACCHARIDE ON CONNEXIN43 PROTEIN EXPRESSION IN RAT HIPPOCAMPUS

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SAYYAH MOHAMMAD; KAVIANI BAHAR; KHOSHKHOLGH SIMA BAHARAK; BAGHERI MARZIEH; OLAD MARYAM; CHOOPANI SAMIRA; MAHDIAN REZA

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Journal: Iranian Biomedical Journal Year:2012 | Volume:16 | Issue:1 Page(s): 25-32

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Title

EFFECT OF CHRONIC INTRACEREBROVENTRICLUAR ADMINISTRATION OF LIPOPOLYSACCHARIDE ON CONNEXIN43 PROTEIN EXPRESSION IN RAT HIPPOCAMPUS

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Keywords

CONNEXIN36 (CX36)

CONNEXIN43 (CX43)

INTERLEUKIN-1 β (IL1-β)

Abstract

Background: Hippocampal damages, which are accompanied by inflammation, are among the main causes of epilepsy acquisition. We previously reported that chronic intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS) modulates epileptogenesis in rats. There is a network of gap junction channels in the hippocampus that contribute to epileptogenesis. Gap junction channels are formed by oligomeric protein subunits called connexins (Cx). Astrocytic Cx43 and neuronal Cx36 are expressed in the hippocampus. In order to find out the possible role of gap junctions in seizure-modulating effect of LPS and neuroinflammation, we studied the effect of central administration of LPS on expression of Cx36 and Cx43 in rat hippocampus.Methods: LPS, 2.5 mg/rat/day, was injected i.c.v. to male Wistar rats for 14 days. M RNA and protein abundance of Cx36, Cx43 and IL1-b were measured in rat hippocampus by real time-PCR, Western blot and ELISA techniques, at the beginning, in the middle, and at the end of the treatment period.Results: IL1-b protein level was significantly increased 6 h after first injection of LPS. Cx36 and Cx43 mRNA expression did not alter during chronic administration of LPS.A selective decrease in Cx43 protein expression was observed after 7 injections of LPS.Conclusion: It is suggested that Cx43 containing gap junctions in the hippocampus is down-regulated in response to chronic injection of LPS. This event can inhibit propagation of toxic and noxious molecules to neighboring cells and modulate hippocampal excitability and epileptogenesis.

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APA: Copy

SAYYAH, MOHAMMAD, KAVIANI, BAHAR, KHOSHKHOLGH SIMA, BAHARAK, BAGHERI, MARZIEH, OLAD, MARYAM, CHOOPANI, SAMIRA, & MAHDIAN, REZA. (2012). EFFECT OF CHRONIC INTRACEREBROVENTRICLUAR ADMINISTRATION OF LIPOPOLYSACCHARIDE ON CONNEXIN43 PROTEIN EXPRESSION IN RAT HIPPOCAMPUS. IRANIAN BIOMEDICAL JOURNAL, 16(1), 25-32. SID. https://sid.ir/paper/277301/en

Vancouver: Copy

SAYYAH MOHAMMAD, KAVIANI BAHAR, KHOSHKHOLGH SIMA BAHARAK, BAGHERI MARZIEH, OLAD MARYAM, CHOOPANI SAMIRA, MAHDIAN REZA. EFFECT OF CHRONIC INTRACEREBROVENTRICLUAR ADMINISTRATION OF LIPOPOLYSACCHARIDE ON CONNEXIN43 PROTEIN EXPRESSION IN RAT HIPPOCAMPUS. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2012;16(1):25-32. Available from: https://sid.ir/paper/277301/en

IEEE: Copy

MOHAMMAD SAYYAH, BAHAR KAVIANI, BAHARAK KHOSHKHOLGH SIMA, MARZIEH BAGHERI, MARYAM OLAD, SAMIRA CHOOPANI, and REZA MAHDIAN, “EFFECT OF CHRONIC INTRACEREBROVENTRICLUAR ADMINISTRATION OF LIPOPOLYSACCHARIDE ON CONNEXIN43 PROTEIN EXPRESSION IN RAT HIPPOCAMPUS,” IRANIAN BIOMEDICAL JOURNAL, vol. 16, no. 1, pp. 25–32, 2012, [Online]. Available: https://sid.ir/paper/277301/en

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