DISTRIBUTION, FAUNA AND SEASONAL VARIATION OF SANDFLIES, SIMULTANEOUS DETECTION OF NUCLEAR INTERNAL TRANSCRIBED SPACER RIBOSOMAL DNA GENE OF LEISHMANIA MAJOR IN RHOMBOMYS OPIMUS AND PHLEBOTOMUS PAPATASI, IN NATANZ DISTRICT IN CENTRAL PART OF IRAN

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PARVIZI PARVIZ; AKHOUNDI MOHAMMAD; MIRZAEI HANIEH

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Journal: Iranian Biomedical Journal Year:2012 | Volume:16 | Issue:2 Page(s): 113-120

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Title

DISTRIBUTION, FAUNA AND SEASONAL VARIATION OF SANDFLIES, SIMULTANEOUS DETECTION OF NUCLEAR INTERNAL TRANSCRIBED SPACER RIBOSOMAL DNA GENE OF LEISHMANIA MAJOR IN RHOMBOMYS OPIMUS AND PHLEBOTOMUS PAPATASI, IN NATANZ DISTRICT IN CENTRAL PART OF IRAN

Author(s)

PARVIZI PARVIZ | AKHOUNDI MOHAMMAD | MIRZAEI HANIEH | Issue Writer Certificate

Keywords

LEISHMANIA MAJOR

SANDFLIES

LEISHMANIASIS

IRAN

Abstract

Background: Zoonotic cutaneous LEISHMANIASIS (ZCL) due to LEISHMANIA MAJOR is increasing in many parts of IRAN. This disease originally is a disease found in gerbils. Leishmania parasites are transmitted by SANDFLIES that live and breed in gerbil burrows. Nested PCR amplified Leishmania ITS1-5.8S rRNA gene in both main reservoir host “Rhombomys opimus” and in the “Phlebotomus papatasi” main vector of ZCL, in IRAN. Population differentiation and seasonal variation of SANDFLIES were analyzed at a microgeographical level in order to identify any isolation by distance, habitat or seasons.Methods: Populations of SANDFLIES were sampled from the edges of villages in Natanz, Isfahan province, IRAN, using the Centers for Disease Control traps and sticky papers. Individual SANDFLIES were identified based on external and internal morphological characters. Nested PCR protocols were used to amplify Leishmania ITS1-5.8S rRNA gene, which were shown to be species-specific via DNA sequence.Results: A total of 4500 SANDFLIES were collected and identified. P. papatasi, Phlebotomus sergenti and Phlebotomus jacusieli from genus Phlebotomus and Sergentomyia sintoni and Sergentomyia clydei from genus Sergentomyia were identified in this region. P. papatasi was the most abundant sandfly in the collections. Ten out of 549 female P. papatasi and four out of 19 R. opimus were found to be infected with L. major.Conclusion: Seasonal activity of SANDFLIES starts in June and ends in November. Abundance of P. papatasi was in September. Finding and molecular typing of L. major in P. papatasi and R. opimus confirmed the main vector and reservoir in this region.

Cites

FAST METHOD FOR DIAGNOSIS OF LEISHMANIA BY PCR AND FLASH PCR

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APA: Copy

PARVIZI, PARVIZ, AKHOUNDI, MOHAMMAD, & MIRZAEI, HANIEH. (2012). DISTRIBUTION, FAUNA AND SEASONAL VARIATION OF SANDFLIES, SIMULTANEOUS DETECTION OF NUCLEAR INTERNAL TRANSCRIBED SPACER RIBOSOMAL DNA GENE OF LEISHMANIA MAJOR IN RHOMBOMYS OPIMUS AND PHLEBOTOMUS PAPATASI, IN NATANZ DISTRICT IN CENTRAL PART OF IRAN. IRANIAN BIOMEDICAL JOURNAL, 16(2), 113-120. SID. https://sid.ir/paper/277331/en

Vancouver: Copy

PARVIZI PARVIZ, AKHOUNDI MOHAMMAD, MIRZAEI HANIEH. DISTRIBUTION, FAUNA AND SEASONAL VARIATION OF SANDFLIES, SIMULTANEOUS DETECTION OF NUCLEAR INTERNAL TRANSCRIBED SPACER RIBOSOMAL DNA GENE OF LEISHMANIA MAJOR IN RHOMBOMYS OPIMUS AND PHLEBOTOMUS PAPATASI, IN NATANZ DISTRICT IN CENTRAL PART OF IRAN. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2012;16(2):113-120. Available from: https://sid.ir/paper/277331/en

IEEE: Copy

PARVIZ PARVIZI, MOHAMMAD AKHOUNDI, and HANIEH MIRZAEI, “DISTRIBUTION, FAUNA AND SEASONAL VARIATION OF SANDFLIES, SIMULTANEOUS DETECTION OF NUCLEAR INTERNAL TRANSCRIBED SPACER RIBOSOMAL DNA GENE OF LEISHMANIA MAJOR IN RHOMBOMYS OPIMUS AND PHLEBOTOMUS PAPATASI, IN NATANZ DISTRICT IN CENTRAL PART OF IRAN,” IRANIAN BIOMEDICAL JOURNAL, vol. 16, no. 2, pp. 113–120, 2012, [Online]. Available: https://sid.ir/paper/277331/en

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