مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

EVALUATION OF NEUROGENIC POTENTIAL OF HUMAN UMBILICAL CORD MESENCHYMAL CELLS, A TIME- AND CONCENTRATION- DEPENDENT MANNER

Pages

  82-90

Abstract

 Background: RETINOIC ACID as one of the most important regulators for CELL DIFFERENTIATION was examined in this study for differentiation of human umbilical mesenchymal cells (hUCM).Methods: After isolation, hUCM were evaluated for mesenchymal stem cell properties by flow cytometry and alkaline phosphatase assay. Also, doubling time of the cells and their differentiation potential into adipogenic and osteogenic cells were tested. hUCM were then cultured with different concentrations of RETINOIC ACID, and on days 1, 7, and 12, the percentage of differentiated cells was determined by immunostaining for nestin, anti-microtubule associated protein 2 (MAP2), glutamic acid decarboxylase (GAD), and gamma-aminobutyric acid (GABA) markers.Results: The isolated cells were negative for the hematopoietic markers and positive for the mesenchymal markers. They showed the population doubling time 60± 3 hours and differentiated into osteogenic and adipogenic cells. A descending trend in nestin and an ascending trend in MAP2, GAD, and GABA expression were observed from the first day until the last day between different concentrations of RETINOIC ACID.Conclusion: hUCM cells may have the potential to differentiate into neural cells in the presence of different incubation period and concentration of RETINOIC ACID.

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    APA: Copy

    EFTEKHAR VAGHEFI, SEYED HASSAN, ZAHMATKESH, LEILA, SALEHINEJAD, PARVIN, TOTONCHI, SHAHIN, & SHAMS ARA, ALI. (2015). EVALUATION OF NEUROGENIC POTENTIAL OF HUMAN UMBILICAL CORD MESENCHYMAL CELLS, A TIME- AND CONCENTRATION- DEPENDENT MANNER. IRANIAN BIOMEDICAL JOURNAL, 19(2), 82-90. SID. https://sid.ir/paper/277344/en

    Vancouver: Copy

    EFTEKHAR VAGHEFI SEYED HASSAN, ZAHMATKESH LEILA, SALEHINEJAD PARVIN, TOTONCHI SHAHIN, SHAMS ARA ALI. EVALUATION OF NEUROGENIC POTENTIAL OF HUMAN UMBILICAL CORD MESENCHYMAL CELLS, A TIME- AND CONCENTRATION- DEPENDENT MANNER. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(2):82-90. Available from: https://sid.ir/paper/277344/en

    IEEE: Copy

    SEYED HASSAN EFTEKHAR VAGHEFI, LEILA ZAHMATKESH, PARVIN SALEHINEJAD, SHAHIN TOTONCHI, and ALI SHAMS ARA, “EVALUATION OF NEUROGENIC POTENTIAL OF HUMAN UMBILICAL CORD MESENCHYMAL CELLS, A TIME- AND CONCENTRATION- DEPENDENT MANNER,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 2, pp. 82–90, 2015, [Online]. Available: https://sid.ir/paper/277344/en

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