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1

Information Journal Paper

Title

FUNCTIONAL RECOMBINANT EXTRA MEMBRANE LOOP OF HUMAN CD20, AN ALTERNATIVE OF THE FULL LENGTH CD20 ANTIGEN

Pages

  121-126

Keywords

Abstract

 Background: Targeting of CD20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-Hodgkin's lymphomas and immunotherapeutic depletion of malignant B cells. Accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. One major problem in expression of full length CD20 is aggregation and misfolding. Therefore, production of an alternative polypeptide is easer and favorable comparing to that of a full length transmembrane protein CD20.Methods: In this study, we expressed the extra membrane loop of hCD20 (exCD20) consisting of a non-glycosylated 47-amino acids region. The exCD20 coding sequence was amplified by PCR and cloned in pET32a (+) expression vector. The desired protein was expressed in fusion with THIOREDOXIN and 6× His tag in E. COLI Origami strain. ELISA and Western blotting data were performed to indicate the functionality of this protein.Results: We have obtained the exCD20 recombinant protein which can be detected in ELISA and Western-blot experiments. This recombinant fusion protein was soluble and stable without aggregation and misfolding problems.Conclusion: The recombinant extra membrane loop of human CD20 protein in fusion with THIOREDOXIN (exCD20) can be used in function assays and some applications such as ELISA, immuneblotting, affinity purification, immunization, screening, and development of anti-CD20 antibodies.

Cites

References

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    • Cite

    APA: Copy

    HABIBI ANBOUHI, MAHDI, FEIZ BARAZANDEH, AIDA, BOUZARI, SAEID, ABOLHASSANI, MOHSEN, KHANAHMAD, HOSSEIN, GOLKAR, MAJID, AGHASADEGHI, MOHAMMAD REZA, BEHDANI, MAHDI, JAHANIAN NAJAFABADI, ALI, & SHOKRGOZAR, MOHAMMAD ALI. (2012). FUNCTIONAL RECOMBINANT EXTRA MEMBRANE LOOP OF HUMAN CD20, AN ALTERNATIVE OF THE FULL LENGTH CD20 ANTIGEN. IRANIAN BIOMEDICAL JOURNAL, 16(3), 121-126. SID. https://sid.ir/paper/277415/en

    Vancouver: Copy

    HABIBI ANBOUHI MAHDI, FEIZ BARAZANDEH AIDA, BOUZARI SAEID, ABOLHASSANI MOHSEN, KHANAHMAD HOSSEIN, GOLKAR MAJID, AGHASADEGHI MOHAMMAD REZA, BEHDANI MAHDI, JAHANIAN NAJAFABADI ALI, SHOKRGOZAR MOHAMMAD ALI. FUNCTIONAL RECOMBINANT EXTRA MEMBRANE LOOP OF HUMAN CD20, AN ALTERNATIVE OF THE FULL LENGTH CD20 ANTIGEN. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2012;16(3):121-126. Available from: https://sid.ir/paper/277415/en

    IEEE: Copy

    MAHDI HABIBI ANBOUHI, AIDA FEIZ BARAZANDEH, SAEID BOUZARI, MOHSEN ABOLHASSANI, HOSSEIN KHANAHMAD, MAJID GOLKAR, MOHAMMAD REZA AGHASADEGHI, MAHDI BEHDANI, ALI JAHANIAN NAJAFABADI, and MOHAMMAD ALI SHOKRGOZAR, “FUNCTIONAL RECOMBINANT EXTRA MEMBRANE LOOP OF HUMAN CD20, AN ALTERNATIVE OF THE FULL LENGTH CD20 ANTIGEN,” IRANIAN BIOMEDICAL JOURNAL, vol. 16, no. 3, pp. 121–126, 2012, [Online]. Available: https://sid.ir/paper/277415/en

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