EXPRESSION ANALYSIS OF MIR-21 AND MIR-221 IN CANCEROUS TISSUES FROM IRANIAN PATIENTS WITH GASTRIC CANCER

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EFFATPANAH HOSEIN; YADEGARAZARI REZA; KARAMI MANOOCHEHR; MAJLESI AMIR; SHABAB NOOSHIN; SAIDIJAM MASSOUD

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Journal: Iranian Biomedical Journal Year:2015 | Volume:19 | Issue:4 Page(s): 188-193

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Title

EXPRESSION ANALYSIS OF MIR-21 AND MIR-221 IN CANCEROUS TISSUES FROM IRANIAN PATIENTS WITH GASTRIC CANCER

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Keywords

MICRORNAS

TUMOR MARKERS

STOMACH NEOPLASMS

Abstract

Background: Early detection is a key to survival for gastric cancer. Molecular markers such as miRNA (microRNA) can have great importance in the early diagnosis of gastric cancer. Expression of miR-21 and miR-221 are deregulated in many types of human cancers. This study aimed to investigate the differences in miRNA expression patterns within the Iranian population. Methods: Total RNA was extracted from gastric cancer tissues and adjacent non-cancerous tissues from 32 patients. Expression levels of miR-21 and miR-221 were detected by Real time RT-PCR using a specific primer, with 5s rRNA as the internal reference gene. Results: Our data showed that the expression levels of miR-21 and miR-221 in gastric cancer samples were significantly higher than in paired non-cancerous samples (P < 0.05). The receiver operating characteristic (ROC) analyses yielded the area under the curve (AUC) values of 80.30 for miR-21 and 93.30 for miR-221, and combined ROC analysis revealed the highest AUC value of 96.90 in discriminating GC patients from healthy controls. Conclusion: It seems that miR-21 and miR-221 expression pattern in Iranian patients with gastric cancer are similar to any other population. Considering the increased expression level of two miRNAs in cancerous tissue compared to normal tissue as well as the area under ROC curve, miR-21 and miR-221 can be used for early detection of gastric cancer.

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APA: Copy

EFFATPANAH, HOSEIN, YADEGARAZARI, REZA, KARAMI, MANOOCHEHR, MAJLESI, AMIR, SHABAB, NOOSHIN, & SAIDIJAM, MASSOUD. (2015). EXPRESSION ANALYSIS OF MIR-21 AND MIR-221 IN CANCEROUS TISSUES FROM IRANIAN PATIENTS WITH GASTRIC CANCER. IRANIAN BIOMEDICAL JOURNAL, 19(4), 188-193. SID. https://sid.ir/paper/277438/en

Vancouver: Copy

EFFATPANAH HOSEIN, YADEGARAZARI REZA, KARAMI MANOOCHEHR, MAJLESI AMIR, SHABAB NOOSHIN, SAIDIJAM MASSOUD. EXPRESSION ANALYSIS OF MIR-21 AND MIR-221 IN CANCEROUS TISSUES FROM IRANIAN PATIENTS WITH GASTRIC CANCER. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(4):188-193. Available from: https://sid.ir/paper/277438/en

IEEE: Copy

HOSEIN EFFATPANAH, REZA YADEGARAZARI, MANOOCHEHR KARAMI, AMIR MAJLESI, NOOSHIN SHABAB, and MASSOUD SAIDIJAM, “EXPRESSION ANALYSIS OF MIR-21 AND MIR-221 IN CANCEROUS TISSUES FROM IRANIAN PATIENTS WITH GASTRIC CANCER,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 4, pp. 188–193, 2015, [Online]. Available: https://sid.ir/paper/277438/en

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