Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling

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payandeh Zahra; RAJABI BAZL MASOUMEH; MORTAZAVI YOUSEF; RAHIMPOUR AZAM; TAROMCHI AMIR HOSSEIN

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2018 | Volume:22 | Issue:3 Page(s): 180-192

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Title

Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling

Author(s)

Keywords

Monoclonal antibody

Ofatumumab

Protein engineering

Abstract

Background: Ofatumumab, an anti-CD20 mAb, was approved in 2009 for the treatment of chronic lymphocytic leukemia. This mAb acts through immune-mediated mechanisms, in particular complement-dependent cytotoxicity and antibody-dependent cellular cytotoxicity by natural killer cells as well as antibody-dependent phagocytosis by macrophages. Apoptosis induction is another mechanism of this antibody. Computational docking is the method of predicting the conformation of an antibody-antigen from its separated elements. Validation of the designed antibodies is carried out by docking tools. Increased affinity enhances the biological action of the antibody, which in turn improves the therapeutic effects. Furthermore, the increased antibody affinity can reduce the therapeutic dose of the antibody, resulting in lower toxicity and handling cost. Methods: Considering the importance of this issue, using in silico analysis such as docking and molecular dynamics, we aimed to find the important amino acids of the Ofatumumab antibody and then replaced these amino acids with others to improve antibody-binding affinity. Finally, we examined the binding affinity of antibody variants to antigen. Results: Our findings showed that variant 3 mutations have improved the characteristics of antibody binding compared to normal Ofatumumab antibodies. Conclusion: The designed anti-CD20 antibodies showed potentiality for improved affinity in comparison to commercial Ofatumumab.

Cites

Structure Evaluation of IroN for Designing a Vaccine against Escherichia Coli, an In Silico Approach

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APA: Copy

payandeh, Zahra, RAJABI BAZL, MASOUMEH, MORTAZAVI, YOUSEF, RAHIMPOUR, AZAM, & TAROMCHI, AMIR HOSSEIN. (2018). Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling. IRANIAN BIOMEDICAL JOURNAL, 22(3), 180-192. SID. https://sid.ir/paper/277482/en

Vancouver: Copy

payandeh Zahra, RAJABI BAZL MASOUMEH, MORTAZAVI YOUSEF, RAHIMPOUR AZAM, TAROMCHI AMIR HOSSEIN. Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2018;22(3):180-192. Available from: https://sid.ir/paper/277482/en

IEEE: Copy

Zahra payandeh, MASOUMEH RAJABI BAZL, YOUSEF MORTAZAVI, AZAM RAHIMPOUR, and AMIR HOSSEIN TAROMCHI, “Ofatumumab Monoclonal Antibody Affinity Maturation Through in silico Modeling,” IRANIAN BIOMEDICAL JOURNAL, vol. 22, no. 3, pp. 180–192, 2018, [Online]. Available: https://sid.ir/paper/277482/en

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