COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE

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ABBASI N.; SHARIF TABRIZI A.; FARZAMI B.; VARDASBI S.; BAHAR M.; ANSARI M.; KHORAMI ZADEH R.; GERAYESH NEJAD S.; KHAGHANI S.

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Journal: ACTA MEDICA IRANICA Year:2007 | Volume:45 | Issue:4 Page(s): 282-284

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Title

COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE

Author(s)

Keywords

LIPOAMIDE DEHYDROGENASE

LYMPHOCYTE

HL-60

LEUKEMIA

Abstract

To determine the importance of Q10H2 as an antioxidant in cancer, we measured the activity of LIPOAMIDE DEHYDROGENASE (Q10H2 recycling enzyme) in HL-60 and normal LYMPHOCYTE. The cultured cells of HL-60 and human normal LYMPHOCYTEs were assayed in cell lysate of given number of both HL- 60 and normal LYMPHOCYTE. The activity of LIPOAMIDE DEHYDROGENASE and the protein concentration were determined by spectrometric methods. The activity of LAD was found to be 0.216.mol/min/.g protein in HL-60 cells and 0.0415.mol/min/.g protein in normal LYMPHOCYTEs. Although the average protein concentration ratio in HL-60 cells to normal LYMPHOCYTE was found to be %124, the average ratio of enzyme activity in HL-60 to normal LYMPHOCYTE was %526. These results are indicative of independence of enzyme increase in HL-60 cells of increase of protein synthesis.

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APA: Copy

ABBASI, N., SHARIF TABRIZI, A., FARZAMI, B., VARDASBI, S., BAHAR, M., ANSARI, M., KHORAMI ZADEH, R., GERAYESH NEJAD, S., & KHAGHANI, S.. (2007). COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE. ACTA MEDICA IRANICA, 45(4), 282-284. SID. https://sid.ir/paper/278025/en

Vancouver: Copy

ABBASI N., SHARIF TABRIZI A., FARZAMI B., VARDASBI S., BAHAR M., ANSARI M., KHORAMI ZADEH R., GERAYESH NEJAD S., KHAGHANI S.. COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE. ACTA MEDICA IRANICA[Internet]. 2007;45(4):282-284. Available from: https://sid.ir/paper/278025/en

IEEE: Copy

N. ABBASI, A. SHARIF TABRIZI, B. FARZAMI, S. VARDASBI, M. BAHAR, M. ANSARI, R. KHORAMI ZADEH, S. GERAYESH NEJAD, and S. KHAGHANI, “COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE,” ACTA MEDICA IRANICA, vol. 45, no. 4, pp. 282–284, 2007, [Online]. Available: https://sid.ir/paper/278025/en

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