CLINICAL GRADE HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL BANKING

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LARIJANI BAGHER; AGHAYAN HAMIDREZA; GOODARZI PARISA; MOHAMADI JAHANI FERESHTEH; NOROUZI JAVIDAN ABBAS; DEHPOUR AHMAD REZA; FALLAHZADEH KHADIJEH; SAYAHPOUR FOROUGH AZAM; BIDAKI KAZEM; ARJMAND BABAK

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Journal: ACTA MEDICA IRANICA Year:2015 | Volume:53 | Issue:9 Page(s): 540-546

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Title

CLINICAL GRADE HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL BANKING

Author(s)

Keywords

ADIPOSE-DERIVED STEM CELL

CELL THERAPY

GOOD MANUFACTURING PRACTICE

MESENCHYMAL STEM CELLS

Abstract

In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived MESENCHYMAL STEM CELLS for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current GOOD MANUFACTURING PRACTICE (cGMP) compliant adipose tissue-derived MESENCHYMAL STEM CELLS from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors’ age was 47.6±8.29 year, mean of cell viability was 95.6±1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106±2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived MESENCHYMAL STEM CELLS preparation and banking for clinical cell transplantation trials.

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APA: Copy

LARIJANI, BAGHER, AGHAYAN, HAMIDREZA, GOODARZI, PARISA, MOHAMADI JAHANI, FERESHTEH, NOROUZI JAVIDAN, ABBAS, DEHPOUR, AHMAD REZA, FALLAHZADEH, KHADIJEH, SAYAHPOUR, FOROUGH AZAM, BIDAKI, KAZEM, & ARJMAND, BABAK. (2015). CLINICAL GRADE HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL BANKING. ACTA MEDICA IRANICA, 53(9), 540-546. SID. https://sid.ir/paper/278805/en

Vancouver: Copy

LARIJANI BAGHER, AGHAYAN HAMIDREZA, GOODARZI PARISA, MOHAMADI JAHANI FERESHTEH, NOROUZI JAVIDAN ABBAS, DEHPOUR AHMAD REZA, FALLAHZADEH KHADIJEH, SAYAHPOUR FOROUGH AZAM, BIDAKI KAZEM, ARJMAND BABAK. CLINICAL GRADE HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL BANKING. ACTA MEDICA IRANICA[Internet]. 2015;53(9):540-546. Available from: https://sid.ir/paper/278805/en

IEEE: Copy

BAGHER LARIJANI, HAMIDREZA AGHAYAN, PARISA GOODARZI, FERESHTEH MOHAMADI JAHANI, ABBAS NOROUZI JAVIDAN, AHMAD REZA DEHPOUR, KHADIJEH FALLAHZADEH, FOROUGH AZAM SAYAHPOUR, KAZEM BIDAKI, and BABAK ARJMAND, “CLINICAL GRADE HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELL BANKING,” ACTA MEDICA IRANICA, vol. 53, no. 9, pp. 540–546, 2015, [Online]. Available: https://sid.ir/paper/278805/en

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