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Information Journal Paper

Title

AUTOLYTIC ACTIVITY AND PLASMA BINDING STUDY OF AAP, A NOVEL MINOR AUTOLYSIN OF STREPTOCOCCUS PNEUMONIA

Pages

  196-200

Abstract

 Pneumococcal autolysins are enzymes involved in cell wall turnover and cellular division physiologically. They have been found to be involved in the pneumococcus pathogenesis. The aim of this study was to identify the autolytic activity of Spr1754 as a novel protein ofSTREPTOCOCCUS PNEUMONIAe.Moreover, the binding of the recombinant protein to plasma proteins was also determined. Thespr1754 gene was amplified by PCR and cloned into thep ET21a (+) prokaryotic expression vector. The constructed pET21a (+) / spr1754 recombinant plasmid was transformed into E. coli Origami (DE3) and induced using IPTG. The recombinant protein of Spr1754 was purified by Ni-NTA affinity chromatography and confirmed by SDS-PAGE and Western blot analysis using anti-His tag monoclonal antibody. Autolytic activity and the ability of the recombinant protein in binding to plasma proteins were performed using zymogram analysis and western blot, respectively. Thespr1754 with expected size was cloned and overexpressed in Escherichia coli Origami (DE3), successfully. After purification of the Spr1754 recombinant protein, the autolytic activity was observed by zymography. Of the four plasma proteins used in this study, binding of lactoferrin to Spr1754 recombinant protein was shown. The Spr1754 recombinant protein has a bifunctional activity, i.e., as being autolysin and lactoferrin binding and designated as Aap (autolytic/ adhesion/ pneumococcus). Nevertheless, characterization of the Aap needs to be followed using gene inactivation and cell wall localization.

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    APA: Copy

    MAHBOOBI, RAMINA, AFSHAR, DAVOUD, POURMAND, MOHAMMAD REZA, & MASHHADI, RAHIL. (2016). AUTOLYTIC ACTIVITY AND PLASMA BINDING STUDY OF AAP, A NOVEL MINOR AUTOLYSIN OF STREPTOCOCCUS PNEUMONIA. ACTA MEDICA IRANICA, 54(3), 196-200. SID. https://sid.ir/paper/278890/en

    Vancouver: Copy

    MAHBOOBI RAMINA, AFSHAR DAVOUD, POURMAND MOHAMMAD REZA, MASHHADI RAHIL. AUTOLYTIC ACTIVITY AND PLASMA BINDING STUDY OF AAP, A NOVEL MINOR AUTOLYSIN OF STREPTOCOCCUS PNEUMONIA. ACTA MEDICA IRANICA[Internet]. 2016;54(3):196-200. Available from: https://sid.ir/paper/278890/en

    IEEE: Copy

    RAMINA MAHBOOBI, DAVOUD AFSHAR, MOHAMMAD REZA POURMAND, and RAHIL MASHHADI, “AUTOLYTIC ACTIVITY AND PLASMA BINDING STUDY OF AAP, A NOVEL MINOR AUTOLYSIN OF STREPTOCOCCUS PNEUMONIA,” ACTA MEDICA IRANICA, vol. 54, no. 3, pp. 196–200, 2016, [Online]. Available: https://sid.ir/paper/278890/en

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