THE USE OF POLYMERASE CHAIN REACTION ASSAY VERSUS CELL CULTURE IN DETECTING NEONATAL CHLAMYDIAL CONJUNCTIVITIS

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RAFIEI TABATABAEI SEDIGHEH; AFJEIEE SEYED ABOLFAZL; FALLAH FATEMEH; TAHAMI ZANJANI NAFISEH; SHIVA FARIDEH; TAVAKKOLY FARD AREZOU; SHAMSHIRI AHMAD REZA; KARIMI ABDOLLAH

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2012 | Volume:15 | Issue:3 Page(s): 171-175

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Title

THE USE OF POLYMERASE CHAIN REACTION ASSAY VERSUS CELL CULTURE IN DETECTING NEONATAL CHLAMYDIAL CONJUNCTIVITIS

Author(s)

Keywords

CELL CULTURE

CHLAMYDIA TRACHOMATIS

OPHTHALMIA NEONATORUM

PCR

Abstract

One of the most common bacterial infections that causes OPHTHALMIA NEONATORUM is CHLAMYDIA TRACHOMATIS, (C. trachomatis). Very few studies have been performed in Iran using both CELL CULTURE and polymerase chain reaction (PCR) methods to determine the prevalence of C. trachomatis as an etiological agent of OPHTHALMIA NEONATORUM. This study aimed to evaluate the prevalence of neonatal chlamydial conjunctivitis (NCC) as diagnosed by both methods in two hospitals in Tehran, Iran.From March 2008 to May 2009, out of 2253 neonates, 241 (10.7%) with clinical findings of conjunctivitis were included in this study. A total of 241 conjunctival swabs were tested by CELL CULTURE (as the gold standard test), PCR, and Giemsa staining.CELL CULTUREs were positive for C. trachomatis in 31 (12.9%) neonates, C. trachomatis was positive in 40 (16.6%) neonates by PCR and 18 (7.5%) by Giemsa staining. The sensitivity of PCR was 100%, whereas Giemsa staining sensitivity was 58.1%.High sensitivity (100%) and specificity (95.7%) of PCR as compared to culture makes it a proper diagnostic method for the detection of C. trachomatis.

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APA: Copy

RAFIEI TABATABAEI, SEDIGHEH, AFJEIEE, SEYED ABOLFAZL, FALLAH, FATEMEH, TAHAMI ZANJANI, NAFISEH, SHIVA, FARIDEH, TAVAKKOLY FARD, AREZOU, SHAMSHIRI, AHMAD REZA, & KARIMI, ABDOLLAH. (2012). THE USE OF POLYMERASE CHAIN REACTION ASSAY VERSUS CELL CULTURE IN DETECTING NEONATAL CHLAMYDIAL CONJUNCTIVITIS. ARCHIVES OF IRANIAN MEDICINE, 15(3), 171-175. SID. https://sid.ir/paper/280757/en

Vancouver: Copy

RAFIEI TABATABAEI SEDIGHEH, AFJEIEE SEYED ABOLFAZL, FALLAH FATEMEH, TAHAMI ZANJANI NAFISEH, SHIVA FARIDEH, TAVAKKOLY FARD AREZOU, SHAMSHIRI AHMAD REZA, KARIMI ABDOLLAH. THE USE OF POLYMERASE CHAIN REACTION ASSAY VERSUS CELL CULTURE IN DETECTING NEONATAL CHLAMYDIAL CONJUNCTIVITIS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2012;15(3):171-175. Available from: https://sid.ir/paper/280757/en

IEEE: Copy

SEDIGHEH RAFIEI TABATABAEI, SEYED ABOLFAZL AFJEIEE, FATEMEH FALLAH, NAFISEH TAHAMI ZANJANI, FARIDEH SHIVA, AREZOU TAVAKKOLY FARD, AHMAD REZA SHAMSHIRI, and ABDOLLAH KARIMI, “THE USE OF POLYMERASE CHAIN REACTION ASSAY VERSUS CELL CULTURE IN DETECTING NEONATAL CHLAMYDIAL CONJUNCTIVITIS,” ARCHIVES OF IRANIAN MEDICINE, vol. 15, no. 3, pp. 171–175, 2012, [Online]. Available: https://sid.ir/paper/280757/en

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