COMPARISONS BETWEEN RT-PCR, REAL-TIME PCR, AND IN VITRO GLOBIN CHAIN SYNTHESIS BY α/β RATIO CALCULATION FOR DIAGNOSIS OF α- FROM β-THALASSEMIA CARRIERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MARYAMI FERESHTEH; MAHDIAN REZA; JAMALI SOMAYEH; KARIMI ARZANANI MOHSEN; KHATAMI SHOHREH; MARYAMI FAHIMEH; BAYAT PARASTOU; SADEGHI SEDIGHE; KARIMIPOUR MORTEZA; ZEINALI SIROUS

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF IRANIAN MEDICINE Year:2013 | Volume:16 | Issue:4 Page(s): 217-220

Download Full-Text

View:

441

Download:

269

Cites:

Information Journal Paper

Title

COMPARISONS BETWEEN RT-PCR, REAL-TIME PCR, AND IN VITRO GLOBIN CHAIN SYNTHESIS BY α/β RATIO CALCULATION FOR DIAGNOSIS OF α- FROM β-THALASSEMIA CARRIERS

Author(s)

Keywords

COMPARATIVE THRESHOLD CYCLE METHOD

GLOBIN CHAIN SYNTHESIS

REAL-TIME PCR

RT-PCR

THALASSEMIA

Abstract

Background: THALASSEMIA, which may be due to point mutations, translocations, and deletions involving the a or b globin gene, is the most prevalent single gene disorder in Iran. This study aims to calculate the a/b ratio in normal cases, a- and b-thalassemia carriers by RT-PCR, REAL-TIME PCR, and in vitro GLOBIN CHAIN SYNTHESIS (GCS) in order to establish the most accurate technique to distinguish between a- and b-thalassemia carriers in suspicious cases.Methods: The a/b ratios were calculated in all samples by RT-PCR, real-time RT-PCR, and in vitro GCS.Results: Using RT-PCR, the ratios were 1.09±0.07 in normal samples, 1.2±0.17 in b-thalassemia, 1.08 ±0.19 in mild a-thalassemia, and 0.96±0.19 in severe a-thalassemia carriers. In real-time RT-PCR, the ratios were 2.21±1.36 in normal samples, 5.12±1.83 in b-thalassemia, 2.88±0.81 in mild a-thalassemia, and 1.18±0.52 in severe a-thalassemia carriers. With GCS, the ratios were 1.03±0.1 in normal samples, 1.9±0.37 in b-thalassemia, 0.8±0.13 in mild a-thalassemia, and 0.59±0.12 in severe a-thalassemia carriers.Conclusion: To determine the most accurate technique, we statistically analyzed the a/b ratios obtained from the three standard methods.The ratio obtained by GCS and REAL-TIME PCR were helpful in distinguishing between a and b carriers in suspicious patients in whom the mutation detection was limited and the risk for offspring was not clear. The use of this technique is more obvious when time is restricted (i.e. during the pregnancy period).

Cites

No record.

References

No record.

Cite

APA: Copy

MARYAMI, FERESHTEH, MAHDIAN, REZA, JAMALI, SOMAYEH, KARIMI ARZANANI, MOHSEN, KHATAMI, SHOHREH, MARYAMI, FAHIMEH, BAYAT, PARASTOU, SADEGHI, SEDIGHE, KARIMIPOUR, MORTEZA, & ZEINALI, SIROUS. (2013). COMPARISONS BETWEEN RT-PCR, REAL-TIME PCR, AND IN VITRO GLOBIN CHAIN SYNTHESIS BY α/β RATIO CALCULATION FOR DIAGNOSIS OF α- FROM β-THALASSEMIA CARRIERS. ARCHIVES OF IRANIAN MEDICINE, 16(4), 217-220. SID. https://sid.ir/paper/280955/en

Vancouver: Copy

MARYAMI FERESHTEH, MAHDIAN REZA, JAMALI SOMAYEH, KARIMI ARZANANI MOHSEN, KHATAMI SHOHREH, MARYAMI FAHIMEH, BAYAT PARASTOU, SADEGHI SEDIGHE, KARIMIPOUR MORTEZA, ZEINALI SIROUS. COMPARISONS BETWEEN RT-PCR, REAL-TIME PCR, AND IN VITRO GLOBIN CHAIN SYNTHESIS BY α/β RATIO CALCULATION FOR DIAGNOSIS OF α- FROM β-THALASSEMIA CARRIERS. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2013;16(4):217-220. Available from: https://sid.ir/paper/280955/en

IEEE: Copy

FERESHTEH MARYAMI, REZA MAHDIAN, SOMAYEH JAMALI, MOHSEN KARIMI ARZANANI, SHOHREH KHATAMI, FAHIMEH MARYAMI, PARASTOU BAYAT, SEDIGHE SADEGHI, MORTEZA KARIMIPOUR, and SIROUS ZEINALI, “COMPARISONS BETWEEN RT-PCR, REAL-TIME PCR, AND IN VITRO GLOBIN CHAIN SYNTHESIS BY α/β RATIO CALCULATION FOR DIAGNOSIS OF α- FROM β-THALASSEMIA CARRIERS,” ARCHIVES OF IRANIAN MEDICINE, vol. 16, no. 4, pp. 217–220, 2013, [Online]. Available: https://sid.ir/paper/280955/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops