مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

SIMULTANEOUS DETECTION OF MYCOPLASMA PNEUMONIAE, MYCOPLASMA HOMINIS AND MYCOPLASMA ARTHRITIDIS IN SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS BY MULTIPLEX PCR

Pages

  345-350

Abstract

 BACKGROUND: It has been recognized that infectious agents, such as different bacteria and viruses, may play a role in the developing of RHEUMATOID ARTHRITIS (RA). Recently, the mycoplasma species has been implicated in the pathogenesis of RA.Aim: The aim of this study was to design a MULTIPLEX PCR for rapid and simultaneous detection of MYCOPLASMA PNEUMONIAE, MYCOPLASMA HOMINIS, and MYCOPLASMA ARTHRITIDIS in the SYNOVIAL FLUID of patients with RHEUMATOID ARTHRITIS (RA).METHODS: A total of 131 SYNOVIAL FLUID (SF) samples from patients with RA were assayed. MYCOPLASMA PNEUMONIAE (ATCC: 29342), M. hominis (native strain), and the synthetic complete genome of M. arthritidis mitogen (MAM) superantigen were used as controls. All SF samples were subjected to DNA extraction separately and MULTIPLEX PCR was performed. The PCR products were confirmed by sequencingRESULTS: The designed MULTIPLEX PCR was able to detect M. pneumoniae, M. hominis, and M. arthritidis in the SF of patients with RA with a frequency of 30 (22.9%), 23 (17.5%) and 13 (9.9%), respectively.CONCLUSION: In this study, the overall detection of the Mycoplasma species in RA patients was 53.4%; thus, we recommend the application of MULTIPLEX PCR assays when searching for a specific anti mycoplasma treatment for RA patients.

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    APA: Copy

    ATAEE, RAMEZAN ALI, GOLMOHAMMADI, REZA, ALISHIRI, GHOLAM HOSSEIN, MIRNEJAD, REZA, NAJAFI, ALI, ESMAEILI, DAVOUD, & JONAIDI JAFARI, NEMATOLLAH. (2015). SIMULTANEOUS DETECTION OF MYCOPLASMA PNEUMONIAE, MYCOPLASMA HOMINIS AND MYCOPLASMA ARTHRITIDIS IN SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS BY MULTIPLEX PCR. ARCHIVES OF IRANIAN MEDICINE, 18(6), 345-350. SID. https://sid.ir/paper/281261/en

    Vancouver: Copy

    ATAEE RAMEZAN ALI, GOLMOHAMMADI REZA, ALISHIRI GHOLAM HOSSEIN, MIRNEJAD REZA, NAJAFI ALI, ESMAEILI DAVOUD, JONAIDI JAFARI NEMATOLLAH. SIMULTANEOUS DETECTION OF MYCOPLASMA PNEUMONIAE, MYCOPLASMA HOMINIS AND MYCOPLASMA ARTHRITIDIS IN SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS BY MULTIPLEX PCR. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2015;18(6):345-350. Available from: https://sid.ir/paper/281261/en

    IEEE: Copy

    RAMEZAN ALI ATAEE, REZA GOLMOHAMMADI, GHOLAM HOSSEIN ALISHIRI, REZA MIRNEJAD, ALI NAJAFI, DAVOUD ESMAEILI, and NEMATOLLAH JONAIDI JAFARI, “SIMULTANEOUS DETECTION OF MYCOPLASMA PNEUMONIAE, MYCOPLASMA HOMINIS AND MYCOPLASMA ARTHRITIDIS IN SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS BY MULTIPLEX PCR,” ARCHIVES OF IRANIAN MEDICINE, vol. 18, no. 6, pp. 345–350, 2015, [Online]. Available: https://sid.ir/paper/281261/en

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