Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes

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NADERI MAHMOOD; PAZOUKI ABDOLREZA; AREFIAN EHSAN; HASHEMI SEYED MAHMOUD; JAMSHIDI ADEGANI FATEMEH; Gholamalamdari Omid; SOUDI SARA; AZADMANESH KAYHAN; MIRAB SAMIEE SIAMAK; MERAT SHAHIN; GHOLAMI FESHARAKI MOHAMMAD; MONDANIZADEH MAHDIEH; VASEI MOHAMMAD; SOLEIMANI MASOUD

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2017 | Volume:20 | Issue:3 Page(s): 165-171

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Title

Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes

Author(s)

Keywords

Lipid metabolism

miR

122

NAFLD

triglycerides

Abstract

BACKGROUND: Expression of miR-122 is highly specific to hepatocytes of the liver. This miRNA is involved in lipid hemostasis of the tissue; however, there is no comprehensive understanding of its function in lipid hemostasis. MATERIALS AND METHODS: Since hepatocytes are responsible for part of Triacylglycerol (TAG) synthesis in the body, we hypothesized that miR-122, as the most abundant miRNA in the tissue, might regulate TAG metabolism by targeting key enzymes that are involved in its production pathway. A systematic computational analysis of putative targets of miR-122 identified CTDNEP1 and LPIN1 genes in the TAG pathway. We used dual-luciferase reporter assay, quantitative RT-PCR as well as western blot to confirm the repressive effect of miR-122 on CTDNEP1 and LPIN1 in TAG pathway. RESULTS: Real time PCR on liver needle biopsies with hepatosteatosis showed that miR-122 is up-regulated in hepatosteatosis. Surprisingly, the protein and RNA level of identified targets of miR-122 are also up-regulated in clinical samples, probably as a disproportionate feedback response to the high level of miR-122. CONCLUSION: Our findings suggest that up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis.

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APA: Copy

NADERI, MAHMOOD, PAZOUKI, ABDOLREZA, AREFIAN, EHSAN, HASHEMI, SEYED MAHMOUD, JAMSHIDI ADEGANI, FATEMEH, Gholamalamdari, Omid, SOUDI, SARA, AZADMANESH, KAYHAN, MIRAB SAMIEE, SIAMAK, MERAT, SHAHIN, GHOLAMI FESHARAKI, MOHAMMAD, MONDANIZADEH, MAHDIEH, VASEI, MOHAMMAD, & SOLEIMANI, MASOUD. (2017). Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes. ARCHIVES OF IRANIAN MEDICINE, 20(3), 165-171. SID. https://sid.ir/paper/281475/en

Vancouver: Copy

NADERI MAHMOOD, PAZOUKI ABDOLREZA, AREFIAN EHSAN, HASHEMI SEYED MAHMOUD, JAMSHIDI ADEGANI FATEMEH, Gholamalamdari Omid, SOUDI SARA, AZADMANESH KAYHAN, MIRAB SAMIEE SIAMAK, MERAT SHAHIN, GHOLAMI FESHARAKI MOHAMMAD, MONDANIZADEH MAHDIEH, VASEI MOHAMMAD, SOLEIMANI MASOUD. Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2017;20(3):165-171. Available from: https://sid.ir/paper/281475/en

IEEE: Copy

MAHMOOD NADERI, ABDOLREZA PAZOUKI, EHSAN AREFIAN, SEYED MAHMOUD HASHEMI, FATEMEH JAMSHIDI ADEGANI, Omid Gholamalamdari, SARA SOUDI, KAYHAN AZADMANESH, SIAMAK MIRAB SAMIEE, SHAHIN MERAT, MOHAMMAD GHOLAMI FESHARAKI, MAHDIEH MONDANIZADEH, MOHAMMAD VASEI, and MASOUD SOLEIMANI, “Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes,” ARCHIVES OF IRANIAN MEDICINE, vol. 20, no. 3, pp. 165–171, 2017, [Online]. Available: https://sid.ir/paper/281475/en

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