USE OF SOME ADDITIVES FOR IMPROVING MESENCHYMAL STEM CELL ISOLATION OUTCOMES IN NON-MOBILIZED PERIPHERAL BLOOD

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POURYAZDANPANAH NOUSHIN; Vahidi Reza; DABIRI SHAHRIAR; DERAKHSHANI ALI; FARSINEJAD ALIREZA

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Journal: ARCHIVES OF IRANIAN MEDICINE Year:2018 | Volume:21 | Issue:8 Page(s): 362-367

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Title

USE OF SOME ADDITIVES FOR IMPROVING MESENCHYMAL STEM CELL ISOLATION OUTCOMES IN NON-MOBILIZED PERIPHERAL BLOOD

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Keywords

DIFFERENTIATION

MESENCHYMAL STEM CELLS

PERIPHERAL BLOOD

REGENERATIVE MEDICINE

Abstract

Background: The MESENCHYMAL STEM CELLS (MSCs) of PERIPHERAL BLOOD (PB) have been recognized as a promising source for allogeneic cell therapy. The objective of the present study was to isolate and characterize MSCs derived from non-mobilized PB, and evaluate their DIFFERENTIATION potential. Methods: The buffy coat mononuclear fractions of the PB were concentrated using the Ficoll-Paque density gradient centrifugation and were grown on primary and secondary culture media, respectively. The isolated cells were characterized using a multidisciplinary approach which was based on morphology, immunophenotyping, gene expression, and multipotentiality. Flow cytometry and Reverse transcription polymerase chain reaction (RT-PCR) were used to identify the expression of different MSC markers. Finally, after culturing in osteogenic and adipogenic induction media, the isolated cells were stained by Alizarin red and Oil-Red O. Results: In spite of absence of any bone marrow stimulating factor, the isolation approach in this study yielded a rather homogeneous and spindle-shaped mononuclear cell population (the yield of passage 0 was 0. 65 ± 0. 15) that stained positive for CD90, CD105, and CD73, and were negative for CD45 and CD34. These cells have high proliferative capacity (confirmed by the expression of Oct-4, Nucleostemin, and Nanog genes) and were able to differentiate into lineage-specific committed cells, when exposed to the appropriate medium. Conclusions: Overall, it can be concluded that conventional, labour-intensive and time-consuming approaches are not necessary in isolating MSCs from PB. This relatively accessible and minimally invasive source, PB, represents a good alternative reservoir of homogeneous MSCs that could open a new era for practical exploitation in REGENERATIVE MEDICINE.

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APA: Copy

POURYAZDANPANAH, NOUSHIN, Vahidi, Reza, DABIRI, SHAHRIAR, DERAKHSHANI, ALI, & FARSINEJAD, ALIREZA. (2018). USE OF SOME ADDITIVES FOR IMPROVING MESENCHYMAL STEM CELL ISOLATION OUTCOMES IN NON-MOBILIZED PERIPHERAL BLOOD. ARCHIVES OF IRANIAN MEDICINE, 21(8), 362-367. SID. https://sid.ir/paper/281564/en

Vancouver: Copy

POURYAZDANPANAH NOUSHIN, Vahidi Reza, DABIRI SHAHRIAR, DERAKHSHANI ALI, FARSINEJAD ALIREZA. USE OF SOME ADDITIVES FOR IMPROVING MESENCHYMAL STEM CELL ISOLATION OUTCOMES IN NON-MOBILIZED PERIPHERAL BLOOD. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2018;21(8):362-367. Available from: https://sid.ir/paper/281564/en

IEEE: Copy

NOUSHIN POURYAZDANPANAH, Reza Vahidi, SHAHRIAR DABIRI, ALI DERAKHSHANI, and ALIREZA FARSINEJAD, “USE OF SOME ADDITIVES FOR IMPROVING MESENCHYMAL STEM CELL ISOLATION OUTCOMES IN NON-MOBILIZED PERIPHERAL BLOOD,” ARCHIVES OF IRANIAN MEDICINE, vol. 21, no. 8, pp. 362–367, 2018, [Online]. Available: https://sid.ir/paper/281564/en

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