Evaluation of the Capability of the Wharton’ s Jelly Mesenchymal Stem Cell Aggregates to Express the Markers of Three Germ Cell Lineages

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAHMANPOUR SOGHRA; TALAEI KHOZANI TAHEREH; Rezaei Tazangi Fatemeh

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF IRANIAN MEDICINE Year:2019 | Volume:22 | Issue:2 Page(s): 85-90

Download Full-Text

View:

321

Download:

138

Cites:

Information Journal Paper

Title

Evaluation of the Capability of the Wharton’ s Jelly Mesenchymal Stem Cell Aggregates to Express the Markers of Three Germ Cell Lineages

Author(s)

BAHMANPOUR SOGHRA | TALAEI KHOZANI TAHEREH | Rezaei Tazangi Fatemeh | Issue Writer Certificate

Keywords

Brachyury

Cell aggregates

Embryoid-body

GATA4

HAND1

Wharton’s jelly

Abstract

Background: The ability of stem cells to differentiate into different cell types makes them a key component of healing damage in regenerative medicine. As human umbilical cord Wharton’ s jelly (HUCWJ) is available non-invasively, HUCWJ does not raise any ethical issues with higher differentiation potential compared to adult stem cells. With the ability to express embryonic stem cell markers, HUCWJ can be considered as a good candidate in regenerative medicine applications. The objective of this study was to find if these cells form Cell aggregates with the same features as that formed by embryonic stem cells (embryoid body) and could form three germ layers. Methods: Eighteen umbilical cords were of healthy infants with parent permission. The umbilical cords were cut into small pieces and the explants were cultured. At the third passage, 1000, 5000 and 10 000 cells/ 20 μ L were cultured in hanging drops for 3 days. Then, they were incubated for additional 3 days in non-adhesive dishes. As the center of Cell aggregates formed from 5000 and 10 000 cells were darker than those formed from 1000 cells, this study focused on the aggregates formed by 1000 cells for further assessments. The immunocytochemistry and flowcytometry were performed using 3 color antibodies to detect the markers for three germ cell lineages. Results: The immunohistochemistry data showed that the Embryoid-body-like aggregates expressed a low amount of ectodermal and endodermal markers and most of the cells expressed mesodermal markers. The flowcytometry percentage of the cells in each aggregate that expressed ectodermal marker Otx2 was17. 1% and endodermal marker, Sox 17 was 5. 49%. The frequency of cells expressing mesodermal marker Brachyury was high (75. 0%). Flowcytometry also showed the percentages by mathematical evaluation and we did this three times for our result accuracy. Conclusion: These aggregates mainly kept their mesenchymal state and showed a poor differentiation potential toward ectoderm and endoderm identity.

Cites

No record.

References

Cite

APA: Copy

BAHMANPOUR, SOGHRA, TALAEI KHOZANI, TAHEREH, & Rezaei Tazangi, Fatemeh. (2019). Evaluation of the Capability of the Wharton’ s Jelly Mesenchymal Stem Cell Aggregates to Express the Markers of Three Germ Cell Lineages. ARCHIVES OF IRANIAN MEDICINE, 22(2), 85-90. SID. https://sid.ir/paper/281816/en

Vancouver: Copy

BAHMANPOUR SOGHRA, TALAEI KHOZANI TAHEREH, Rezaei Tazangi Fatemeh. Evaluation of the Capability of the Wharton’ s Jelly Mesenchymal Stem Cell Aggregates to Express the Markers of Three Germ Cell Lineages. ARCHIVES OF IRANIAN MEDICINE[Internet]. 2019;22(2):85-90. Available from: https://sid.ir/paper/281816/en

IEEE: Copy

SOGHRA BAHMANPOUR, TAHEREH TALAEI KHOZANI, and Fatemeh Rezaei Tazangi, “Evaluation of the Capability of the Wharton’ s Jelly Mesenchymal Stem Cell Aggregates to Express the Markers of Three Germ Cell Lineages,” ARCHIVES OF IRANIAN MEDICINE, vol. 22, no. 2, pp. 85–90, 2019, [Online]. Available: https://sid.ir/paper/281816/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops