SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN A THREE-DIMENSIONAL CULTURE OF HUMAN ENDOMETRIUM; AN IN-VITRO MODEL FOR ENDOMETRIOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AI J.; ESFANDIARI N.; CASPER R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Reproduction & Infertility Year:2009 | Volume:10 | Issue:2 (39) Page(s): 95-100

Download Full-Text

Persian Verion

View:

1,656

Download:

Cites:

Information Journal Paper

Title

SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN A THREE-DIMENSIONAL CULTURE OF HUMAN ENDOMETRIUM; AN IN-VITRO MODEL FOR ENDOMETRIOSIS

Author(s)

AI J. | ESFANDIARI N. | CASPER R. | Issue Writer Certificate

Keywords

ANGIOGENESISQ2

ENDOMETRIOSISQ1

ENDOMETRIUMQ1

FEMALE INFERTILITYQ1

THREE-DIMENSIONAL TISSUE CULTUREQ2

VEGFQ1

Abstract

Introduction: ENDOMETRIOSIS is the presence of endometrial glandular and stromal cells outside the uterine cavity. The disease is prevalent in about 10% of women of reproductive age and in up to 50% of infertile women. Surgery continues to be the first line of treatment in eradicating endometriotic lesions but recurrence of the condition is seen in up to 47% of the cases. VEGF is an effective factor in the establishment of ENDOMETRIOSIS. The aim of the present study was to determine VEGF levels in a three-dimensional (3D) fibrin matrix culture of human endometrial tissue.Materials and Methods: Endometrial biopsies were obtained from the uterine fundus of 10 ovulating (at 19th to 24th day of menstruation cycle) premenopausal women attending the Toronto Center for Advanced Reproductive Technology (TCART) for the treatment of infertility, ovarian cysts or non-endometrial complaints. Each tissue fragment was divided into ten segments for culture in a total of 100 wells. The samples were cultured on three-dimensional fibrin matrices and the supernatant fluid was collected from each endometrial sample for the assessment of VEGF levels. The endometrial samples were stained by anti-Cox-2 antibody (anti-cyclooxygenase -2) for immunohistochemical evaluation of ANGIOGENESIS.Results: VEGF levels in the supernatant fluid of wells with ANGIOGENESIS was significantly higher (p<0.05) than wells with no signs of the phenomenon (492±3.11 and 183±13.2, respectively). The results were indicative of cell proliferation in 91% of the wells and ANGIOGENESIS was observed in 51 wells (56%) with cell proliferation.Conclusion: It seems that VEGF secretion plays an important role in promoting neovasculariza-tion and cell proliferation of endometrial cells. Therefore, VEGF secretion seems to be involved in the establishment of ENDOMETRIOSIS. Seeking the relationship between cell proliferation and VEGF secretion rates is suggested.

Cites

LETTER TO EDITOR

References

Cite

APA: Copy

AI, J., ESFANDIARI, N., & CASPER, R.. (2009). SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN A THREE-DIMENSIONAL CULTURE OF HUMAN ENDOMETRIUM; AN IN-VITRO MODEL FOR ENDOMETRIOSIS. JOURNAL OF REPRODUCTION AND INFERTILITY, 10(2 (39)), 95-100. SID. https://sid.ir/paper/28354/en

Vancouver: Copy

AI J., ESFANDIARI N., CASPER R.. SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN A THREE-DIMENSIONAL CULTURE OF HUMAN ENDOMETRIUM; AN IN-VITRO MODEL FOR ENDOMETRIOSIS. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2009;10(2 (39)):95-100. Available from: https://sid.ir/paper/28354/en

IEEE: Copy

J. AI, N. ESFANDIARI, and R. CASPER, “SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN A THREE-DIMENSIONAL CULTURE OF HUMAN ENDOMETRIUM; AN IN-VITRO MODEL FOR ENDOMETRIOSIS,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 10, no. 2 (39), pp. 95–100, 2009, [Online]. Available: https://sid.ir/paper/28354/en

Related Journal Papers