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Information Journal Paper

Title

INDUCED MUTATION AND IN VITRO TECHNIQUES AS A METHOD TO INDUCE SALT TOLERANCE IN BASMATI RICE (ORYZA SATIVA L.)

Pages

  141-145

Abstract

 Embryogenic CALLUS of indica RICE (Oryza sativa L.) cv. Basmati 370 induced on MS medium containing 9.05 ...M 2, 4-D was irradiated at 50 Gy of gamma rays of 60Co for creating genetic variability against salinity. Irradiated and non-irradiated CALLUSes were screened in vitro through three consecutive proliferation phases at 4.0, 6.0, 8.0 and 10.0 d/Sm electrical conductivity of NaCl. Growth value and number of adapted mutagenized CALLUS was more than that of non-mutagenized CALLUS. Salinity levels beyond 6 d/Sm and 8 d/Sm were lethal to growth and adaptation of non-irradiated and irradiated CALLUS respectively. NaCl adapted irradiated CALLUS showed 2.0%-4.75% REGENERATION frequency on MS REGENERATION medium containing 5.37 M NAA and 9.29 ...M Kinetin. Non-mutagenized salt adapted CALLUS did not show any REGENERATION. From gamma ray mutagenized cultures, 2 putative lines (M2 generation) with moderate salt tolerance were obtained at seedling stage. The results suggest that in vitro technique in connection with gamma rays may be used as a versatile approach to improve the level of salt tolerance in Basmati RICE for saline environment

Cites

References

Cite

APA: Copy

SALEEM, M.Y., CHEEMA, A.A., ATTA, B.M., & MUKHTAR, Z.. (2005). INDUCED MUTATION AND IN VITRO TECHNIQUES AS A METHOD TO INDUCE SALT TOLERANCE IN BASMATI RICE (ORYZA SATIVA L.) . INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), 2(2), 141-145. SID. https://sid.ir/paper/284908/en

Vancouver: Copy

SALEEM M.Y., CHEEMA A.A., ATTA B.M., MUKHTAR Z.. INDUCED MUTATION AND IN VITRO TECHNIQUES AS A METHOD TO INDUCE SALT TOLERANCE IN BASMATI RICE (ORYZA SATIVA L.) . INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST)[Internet]. 2005;2(2):141-145. Available from: https://sid.ir/paper/284908/en

IEEE: Copy

M.Y. SALEEM, A.A. CHEEMA, B.M. ATTA, and Z. MUKHTAR, “INDUCED MUTATION AND IN VITRO TECHNIQUES AS A METHOD TO INDUCE SALT TOLERANCE IN BASMATI RICE (ORYZA SATIVA L.) ,” INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (IJEST), vol. 2, no. 2, pp. 141–145, 2005, [Online]. Available: https://sid.ir/paper/284908/en

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