DEVELOPMENT OF [201TL](III) OXINATE COMPLEX FOR IN VITRO CELL LABELING

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JALILIAN A.R.; HAKIMI A.; GAROUSI J.; BOLOURINOVIN F.; KAMALI DEHGHAN M.; ASLANI G.

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Journal Paper

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Journal: International Journal of Radiation Research Year:2008 | Volume:6 | Issue:3 Page(s): 145-150

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Title

DEVELOPMENT OF [201TL](III) OXINATE COMPLEX FOR IN VITRO CELL LABELING

Author(s)

Keywords

201

SANS-SERIF; COLOR: BLACK">TL(III)

COMPLEX

OXINATE

CELL LABELING

HPLC

SPECT

Abstract

Background: The incorporation of thallium-201 into 8-hydroxyquinoline was targeted for CELL LABELING due to interesting physical properties and wide availability of this nuclide as a single photon emission computed tomography (SPECT) radionuclide. Materials and Methods: Thallium-201 (T1/2=3.04 d) in Tl+ form was converted to Tl3+ cation in presence of O3/6M HCl and di-isopropyl ether, controlled by radiothin layer chromatography (RTLC) /gel electrophoresis methods. The final evaporated activity reacted with ethanolic 8-hydroxy-quinoline (oxine) solution in normal saline to yield [201Tl](III)oxinate at room temperature after 0.5 h, followed by solid phase extraction/purification using C18 Sep-Pak column and partition coefficient determination for water/lipid solubility. In vitro red blood cell (RBC) labeling was also performed. Results: A radiochemical yield of more than 95% was obtained. Radiochemical purity of 92% was obtained using RTLC (>90% using HPLC) with specific activity of about 820 GBq/mmol. The tracer was stable in the final product and in presence of human serum at 37°C up to 6h. The partition coefficient of lopP=5.5 was obtained. The labeled compound was used in RBC labeling. The cell uptake ratio was 0.47 after 240 min. Conclusion: [201Tl](III) OXINATE used in this study is a widely available agent for use in RBC labeling studies in biology, medicine and various other research areas.

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APA: Copy

JALILIAN, A.R., HAKIMI, A., GAROUSI, J., BOLOURINOVIN, F., KAMALI DEHGHAN, M., & ASLANI, G.. (2008). DEVELOPMENT OF [²⁰¹TL](III) OXINATE COMPLEX FOR IN VITRO CELL LABELING. INTERNATIONAL JOURNAL OF RADIATION RESEARCH, 6(3), 145-150. SID. https://sid.ir/paper/286458/en

Vancouver: Copy

JALILIAN A.R., HAKIMI A., GAROUSI J., BOLOURINOVIN F., KAMALI DEHGHAN M., ASLANI G.. DEVELOPMENT OF [²⁰¹TL](III) OXINATE COMPLEX FOR IN VITRO CELL LABELING. INTERNATIONAL JOURNAL OF RADIATION RESEARCH[Internet]. 2008;6(3):145-150. Available from: https://sid.ir/paper/286458/en

IEEE: Copy

A.R. JALILIAN, A. HAKIMI, J. GAROUSI, F. BOLOURINOVIN, M. KAMALI DEHGHAN, and G. ASLANI, “DEVELOPMENT OF [²⁰¹TL](III) OXINATE COMPLEX FOR IN VITRO CELL LABELING,” INTERNATIONAL JOURNAL OF RADIATION RESEARCH, vol. 6, no. 3, pp. 145–150, 2008, [Online]. Available: https://sid.ir/paper/286458/en

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