HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF METHOTREXATE IN PLASMA

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MOGHBEL A.A.H.; ZAND MOGHADAM A.; REZAEI SAEID; PEDRAM MOHAMMAD

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2003 | Volume:2 | Issue:3 Page(s): 149-152

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Information Journal Paper

Title

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF METHOTREXATE IN PLASMA

Author(s)

MOGHBEL A.A.H. | ZAND MOGHADAM A. | REZAEI SAEID | PEDRAM MOHAMMAD | Issue Writer Certificate

Keywords

METHOTREXATE

HPLC

PLASMA CONCENTRATION

Abstract

This article describes a simple and fast high-performance liquid chromatography method for the determination of METHOTREXATE (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was injected into a 250×4.6 mm octadecylsilane column. Mobile phase was made of TRIS-phosphate buffer (pH 5.7): methanol: acetonitrile (82:11:7) with a flow rate of 1.8 ml/min. Ultraviolet detection was done at 313 nm and at ambient temperature. Para-aminoacetophenone was used as internal standard. METHOTREXATE and internal standard retention times were 4.4 and 6.5 minutes, respectively. Results showed that reproducibility (precision) of method within a day was 2.6 to 6 percent and between days was 5.5 to 9.5 percent. The recovery of the method was between 61.5 and 72.7 percent. The quantitation limit of the method for METHOTREXATE was 0.1 mM. This method is suitable for quantitation of METHOTREXATE after infusion of high doses of this drug and has good accuracy, precision and quantiatation limit.

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APA: Copy

MOGHBEL, A.A.H., REZAEI, SAEID, PEDRAM, MOHAMMAD, & ZAND MOGHADAM, A.. (2003). HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF METHOTREXATE IN PLASMA. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 2(3), 149-152. SID. https://sid.ir/paper/286971/en

Vancouver: Copy

MOGHBEL A.A.H., REZAEI SAEID, PEDRAM MOHAMMAD, ZAND MOGHADAM A.. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF METHOTREXATE IN PLASMA. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2003;2(3):149-152. Available from: https://sid.ir/paper/286971/en

IEEE: Copy

A.A.H. MOGHBEL, SAEID REZAEI, MOHAMMAD PEDRAM, and A. ZAND MOGHADAM, “HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF METHOTREXATE IN PLASMA,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 2, no. 3, pp. 149–152, 2003, [Online]. Available: https://sid.ir/paper/286971/en

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