LYSOSOMAL OXIDATIVE STRESS CYTOTOXICITY INDUCED BY PARA-PHENYLENEDIAMINE REDOX CYCLING IN HEPATOCYTES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

POURAHMAD J.; KOBARFARD F.; SHAKOURI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2004 | Volume:3 | Issue:4 Page(s): 193-199

Download Full-Text

View:

331

Download:

140

Cites:

Information Journal Paper

Title

LYSOSOMAL OXIDATIVE STRESS CYTOTOXICITY INDUCED BY PARA-PHENYLENEDIAMINE REDOX CYCLING IN HEPATOCYTES

Author(s)

POURAHMAD J. | KOBARFARD F. | SHAKOURI A. | Issue Writer Certificate

Keywords

DURENEDIAMINE

PHENYENEDIAMINES

OXIDATIVE STRESS

LYSOSOME

HEPATOCYTES

Abstract

It has already been reported that muscle necrosis induced by various phenylenediamine derivatives are correlated with their autoxidation rate. Now in a more detailed investigation of the cytotoxic mechanism using a model system of isolated HEPATOCYTES and ring-methylated structural isomer DURENEDIAMINE (DD) we have shown that under aerobic conditions, phenylenediamine induced cytotoxicity and ROS formation were markedly increased by inactivating. DT-diaphorase but were prevented by a subtoxic concentration of the mitochondrial respiratory inhibitor cyanide. This suggests that the H2O2 generation could be attributed to a futile two electron redox cycle involving oxidation of phenylenediamine to the corresponding diimine by the mitochondrial electron transfer chain and re-reduction by the DT- diaphorase. The subcellular organelle OXIDATIVE STRESS effects leading to cytotoxicity has not yet been identified. Hepatocyte mitochondrial membrane potential was only slightly decreased by phenylenediamine before cytotoxicity ensued. However phenylenediamine induced lysosomal damage and hepatocyte protease activation. Endocytosis inhibitors, lysosomotropic agents or lysosomal protease inhibitors also prevented phenylenediamine induced cytotoxicity.Furthermore desferoxamine (a ferric chelator), antioxidants or ROS scavengers (catalase, mannitol, tempol or dimethylsulfoxide) prevented phenylenediamine cytotoxicity. It is concluded that H2O2 reacts with lysosomal Fe2+ to form "ROS" which causes lysosomal lipid peroxidation, membrane disruption; protease release and cell death.

Cites

No record.

References

No record.

Cite

APA: Copy

POURAHMAD, J., KOBARFARD, F., & SHAKOURI, A.. (2004). LYSOSOMAL OXIDATIVE STRESS CYTOTOXICITY INDUCED BY PARA-PHENYLENEDIAMINE REDOX CYCLING IN HEPATOCYTES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 3(4), 193-199. SID. https://sid.ir/paper/287509/en

Vancouver: Copy

POURAHMAD J., KOBARFARD F., SHAKOURI A.. LYSOSOMAL OXIDATIVE STRESS CYTOTOXICITY INDUCED BY PARA-PHENYLENEDIAMINE REDOX CYCLING IN HEPATOCYTES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2004;3(4):193-199. Available from: https://sid.ir/paper/287509/en

IEEE: Copy

J. POURAHMAD, F. KOBARFARD, and A. SHAKOURI, “LYSOSOMAL OXIDATIVE STRESS CYTOTOXICITY INDUCED BY PARA-PHENYLENEDIAMINE REDOX CYCLING IN HEPATOCYTES,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 3, no. 4, pp. 193–199, 2004, [Online]. Available: https://sid.ir/paper/287509/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops