THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS

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FARZAMFAR B.; BAYAN ALHAGH S.; MAHBOUDI F.; ZAHRAEI M.

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2007 | Volume:6 | Issue:3 Page(s): 179-184

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Title

THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS

Author(s)

FARZAMFAR B. | BAYAN ALHAGH S. | MAHBOUDI F. | ZAHRAEI M. | Issue Writer Certificate

Keywords

AFLATOXIN

BSA

CONJUGATE

HRP

IMMUNOASSAY

STABILIZER

Abstract

AFLATOXINs are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of AFLATOXINs. Preparation and stabilizing of a proper CONJUGATEd tracer for detection of AFLATOXINs is probably the main step for designing an ELISA method. In current study, different STABILIZERs were applied to stabilize a newly prepared CONJUGATEd molecule, Horseradish peroxidase-Bovine serum albumin-AFLATOXIN B1 (HRP-BSA-AFB1). Stabilizing effects of six different STABILIZERs were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing STABILIZERs especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 CONJUGATEd tracer.

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APA: Copy

FARZAMFAR, B., BAYAN ALHAGH, S., MAHBOUDI, F., & ZAHRAEI, M.. (2007). THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 6(3), 179-184. SID. https://sid.ir/paper/287642/en

Vancouver: Copy

FARZAMFAR B., BAYAN ALHAGH S., MAHBOUDI F., ZAHRAEI M.. THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2007;6(3):179-184. Available from: https://sid.ir/paper/287642/en

IEEE: Copy

B. FARZAMFAR, S. BAYAN ALHAGH, F. MAHBOUDI, and M. ZAHRAEI, “THE EFFECT OF DIFFERENT STABILIZERS ON STABILITY OF HORSERADISH PEROXIDASE- BOVINE SERUM ALBUMIN-AFLATOXIN B1, A CONJUGATED TRACER FOR DETECTION OF AFLATOXIN B1 IN IMMUNOASSAY-BASED METHODS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 6, no. 3, pp. 179–184, 2007, [Online]. Available: https://sid.ir/paper/287642/en

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