مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

550
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

492
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ESTIMATION OF DROTAVERINE HYDROCHLORIDE AND MEFENAMIC ACID IN HUMAN PLASMA

Pages

  209-215

Abstract

 The present work describes a reverse phase HPLC method for the quantitation of DROTAVERINE HYDROCHLORIDE and MEFENAMIC ACID in human PLASMA. Organic solvent system used for liquid extraction composed of dichloromethane, and isopropyl alcohol in the ratio 80:20 (v/v). The compounds were separated on a Thermo BDS Hypersil C8 (25.0 cm×4.6 mm, 5 mm particle size) column in isocratic mode with a mixture of acetonitrile and ammonium acetate buffer (20 mM, pH 3.5 ± 0.05 adjusted with 85% phosphoric acid) in a ratio of 55: 45 (v/v), as the mobile phase, at a flow rate of 1.0 mL min-1. The effluent was monitored by UV detection at 230 nm. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The total chromatographic analysis time was approximately 10 min with diclofenac sodium (internal standard), DROTAVERINE HYDROCHLORIDE and MEFENAMIC ACID, eluting with retention times of 6.89, 8.21 and 9.67 min, respectively. Calibration plots were linear within the range of 32-960 ng mL-1 and 100-3000 ng mL-1 for DROTAVERINE HYDROCHLORIDE and MEFENAMIC ACID, respectively. The limit of detection values were 11 and 33 ng mL-1 and the limit of quantitation values were 32 and 100 ng mL-1 for DROTAVERINE HYDROCHLORIDE and MEFENAMIC ACID, respectively. Results from analysis of quality control samples at concentrations of 90, 450 and 750 ng mL-1 for DROTAVERINE HYDROCHLORIDE and 300, 1600 and 2400 ng mL-1 for MEFENAMIC ACID, were indicative of good repeatability and precision. Recovery from PLASMA was 98.68% for DROTAVERINE HYDROCHLORIDE and 94.51% for MEFENAMIC ACID.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    DAHIVELKAR, P.P., BARI, S.B., BHOIR, S., & BHAGWAT, A.M.. (2009). HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ESTIMATION OF DROTAVERINE HYDROCHLORIDE AND MEFENAMIC ACID IN HUMAN PLASMA. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 8(3), 209-215. SID. https://sid.ir/paper/287730/en

    Vancouver: Copy

    DAHIVELKAR P.P., BARI S.B., BHOIR S., BHAGWAT A.M.. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ESTIMATION OF DROTAVERINE HYDROCHLORIDE AND MEFENAMIC ACID IN HUMAN PLASMA. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2009;8(3):209-215. Available from: https://sid.ir/paper/287730/en

    IEEE: Copy

    P.P. DAHIVELKAR, S.B. BARI, S. BHOIR, and A.M. BHAGWAT, “HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ESTIMATION OF DROTAVERINE HYDROCHLORIDE AND MEFENAMIC ACID IN HUMAN PLASMA,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 8, no. 3, pp. 209–215, 2009, [Online]. Available: https://sid.ir/paper/287730/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button