مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

363
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

159
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DETERMINATION OF L- ASCORBIC ACID IN PLASMA BY VOLTAMMETRIC METHOD

Pages

  123-128

Abstract

 Voltammetric techniques have been considered as important methods among the analytical techniques used for the identification and determination of trace concentrations of many biological molecules such as L-ascorbic acid (AA). L-ascorbic acid is an electro-active molecule, though it is difficult to determine its value directly with a majority of electrodes made of carbon and transition metals, because of electrode surface problems. The present study is based on I-E curves for AA analysis at various pH. Furthermore, the effects of the presence of other electro-active substances; such as COPPER, as well as the effect of the sweep rate of potential will be studied.The present study is based on analysis of the current-voltage curves for L-ascorbic acid at varying pH and sweep rate scan values. An analysis was also carried out to measure the influence of the concentration of some electro active species. The peak height of the first oxidation wave is used for L-ascorbic acid assay. L-ascorbic acid was determined in aqueous media by linear-scan VOLTAMMETRY on a GOLD ELECTRODE; ranging between (1-175 mg/mL). In biologic samples, for elimination of uric acid or some sugars and effects, a significant interference of COPPER ions whose presence reduces the height of the L-ascorbic acid oxidation peak was used. The optimum pH and sweep rate were 3.2 and 7500mV/s, respectively. Under these conditions, the detection limit of the method was 0.3 mg/mL. Repeatability of the method based on relative standard deviation (RSD) 50, 10 and 1 mg/mL concentrations was 0.83, 2.1 and 10.3%, respectively. The calibration curve was linear over the range 1-175 mg/mL (r2 = 0.9977, p < 0.001).The advantage of this method lies in the fact that the use of COPPER eliminates the interference of different substances such as uric acid.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    BEHFAR, A.A.A., SADEGHI, NAFISEH, JANAT, B., & OVEYSI, M.R.. (2010). DETERMINATION OF L- ASCORBIC ACID IN PLASMA BY VOLTAMMETRIC METHOD. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 9(2), 123-128. SID. https://sid.ir/paper/287752/en

    Vancouver: Copy

    BEHFAR A.A.A., SADEGHI NAFISEH, JANAT B., OVEYSI M.R.. DETERMINATION OF L- ASCORBIC ACID IN PLASMA BY VOLTAMMETRIC METHOD. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2010;9(2):123-128. Available from: https://sid.ir/paper/287752/en

    IEEE: Copy

    A.A.A. BEHFAR, NAFISEH SADEGHI, B. JANAT, and M.R. OVEYSI, “DETERMINATION OF L- ASCORBIC ACID IN PLASMA BY VOLTAMMETRIC METHOD,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 9, no. 2, pp. 123–128, 2010, [Online]. Available: https://sid.ir/paper/287752/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button