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Information Journal Paper

Title

EXPRESSION OF AN INNATE IMMUNE ELEMENT (MOUSE HEPCIDIN-1) IN BACULOVIRUS EXPRESSION SYSTEM AND THE COMPARISON OF ITS FUNCTION WITH SYNTHETIC HUMAN HEPCIDIN-25

Pages

  559-568

Abstract

 HEPCIDIN is an innate immune element which decreases the iron absorption from diet and iron releasing from macrophage cell. In contrast to the chemical iron chelators, there has been limited effort applied to the specific use of HEPCIDIN as a new drug for decreasing the iron overload.HEPCIDIN is produced in different biological systems. For instance, E-coli is used for human HEPCIDIN expression, however, post-translational modification is impaired. We have used a simple BACULOVIRUS EXPRESSION SYSTEM (BES) to improve the HEPCIDIN folding and activity. HEPCIDIN Messenger Ribonucleic acid (mRNA) was isolated from mouse liver cells and its complementary Deoxyribonucleic acid (cDNA) was produced and amplified. PFastBac HTB vector was used for recombinant bacmid production. Recombinant baculovirus was produced using SF-9 cell line. The mouse HEPCIDIN-1 protein was expressed in a large quantity and functional tests were performed for this recombinant peptide. The yield of HEPCIDIN in BES was 20 mg/mL and anti-histidine (anti-His) tag antibody was used for the confirmation of HEPCIDIN on western blot nitrocellulose paper. Functional tests showed that mouse HEPCIDIN accumulates iron in the macrophage cell line J774A.1 up to 63%. In addition, our data showed that the mouse HEPCIDIN-1 has less toxicity compared to the synthetic human HEPCIDIN-25 (p=0.000).

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    APA: Copy

    YAZDANI, YAGHOUB, SADEGHI, HAMID, ALIMOHAMMADIAN, MOHAMMAD, ANDALIB, ALIREZA, & MOAZEN, FATEMEH. (2011). EXPRESSION OF AN INNATE IMMUNE ELEMENT (MOUSE HEPCIDIN-1) IN BACULOVIRUS EXPRESSION SYSTEM AND THE COMPARISON OF ITS FUNCTION WITH SYNTHETIC HUMAN HEPCIDIN-25. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 10(3), 559-568. SID. https://sid.ir/paper/287860/en

    Vancouver: Copy

    YAZDANI YAGHOUB, SADEGHI HAMID, ALIMOHAMMADIAN MOHAMMAD, ANDALIB ALIREZA, MOAZEN FATEMEH. EXPRESSION OF AN INNATE IMMUNE ELEMENT (MOUSE HEPCIDIN-1) IN BACULOVIRUS EXPRESSION SYSTEM AND THE COMPARISON OF ITS FUNCTION WITH SYNTHETIC HUMAN HEPCIDIN-25. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2011;10(3):559-568. Available from: https://sid.ir/paper/287860/en

    IEEE: Copy

    YAGHOUB YAZDANI, HAMID SADEGHI, MOHAMMAD ALIMOHAMMADIAN, ALIREZA ANDALIB, and FATEMEH MOAZEN, “EXPRESSION OF AN INNATE IMMUNE ELEMENT (MOUSE HEPCIDIN-1) IN BACULOVIRUS EXPRESSION SYSTEM AND THE COMPARISON OF ITS FUNCTION WITH SYNTHETIC HUMAN HEPCIDIN-25,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 10, no. 3, pp. 559–568, 2011, [Online]. Available: https://sid.ir/paper/287860/en

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