A RAPID, SIMPLE, LIQUID CHROMATOGRAPHIC-ELECTROSPRAY IONIZATION, ION TRAP MASS SPECTROMETRY METHOD FOR THE DETERMINATION OF FINASTERIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEMI MOUSAVI SYED HUSAIN; KOBARFARD FARZAD; HUSAIN SYED WAQIF; TEHRANI MOHAMMAD SABER; ABROOMAND AZAR PARVIZ; AHMADKHANIHA REZA; MEHDIZADEH AMIR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2012 | Volume:11 | Issue:1 Page(s): 59-67

Download Full-Text

View:

312

Download:

136

Cites:

Information Journal Paper

Title

A RAPID, SIMPLE, LIQUID CHROMATOGRAPHIC-ELECTROSPRAY IONIZATION, ION TRAP MASS SPECTROMETRY METHOD FOR THE DETERMINATION OF FINASTERIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY

Author(s)

Keywords

ELECTROSPRAY IONIZATION (ESI)

FINASTERIDE

ION TRAP

LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY (LC–MS)

PHARMACOKINETIC

Abstract

A fast, accurate, sensitive, selective and reliable method using reversed-phase high performance liquid chromatography coupled to electrospray ionization ION TRAP mass spectrometry was developed and validated for the determination of FINASTERIDE in human plasma. After protein precipitation with perchloric acid, satisfactory separation was achieved on a Zorbax Eclipse® C8 analytical column using a mobile phase consisted of acetonitrile, 2 mM ammonium formate buffer (58: 42, pH adjusted at 2.5 using formic acid); the flow rate was 0.25 mLmin-1 and the column oven was set to 50oC. Tamoxifen citrate was used as internal standard. This method involved the use of [M+H]+ ions of FINASTERIDE and IS at m / z 373 and 372 respectively with the selected ion monitoring (SIM) mode. The calibration curve was linear over the range of 0.1–60 ng mL-1. The limit of quantification for FINASTERIDE in plasma was 0.1 ng mL-1. The intra-day and inter-day repeatability (precision) were 2.68-13.87% and 2.14-14.69% respectively. Intra-day and inter-day accuracy were 98-101.57% and 99.7-110%. The assay method has been successfully used to estimate the PHARMACOKINETICs of FINASTERIDE after oral administration of a 5 mg tablet of FINASTERIDE in 12 healthy volunteers.

Cites

No record.

References

No record.

Cite

APA: Copy

HASHEMI MOUSAVI, SYED HUSAIN, KOBARFARD, FARZAD, HUSAIN, SYED WAQIF, TEHRANI, MOHAMMAD SABER, ABROOMAND AZAR, PARVIZ, AHMADKHANIHA, REZA, & MEHDIZADEH, AMIR. (2012). A RAPID, SIMPLE, LIQUID CHROMATOGRAPHIC-ELECTROSPRAY IONIZATION, ION TRAP MASS SPECTROMETRY METHOD FOR THE DETERMINATION OF FINASTERIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 11(1), 59-67. SID. https://sid.ir/paper/287931/en

Vancouver: Copy

HASHEMI MOUSAVI SYED HUSAIN, KOBARFARD FARZAD, HUSAIN SYED WAQIF, TEHRANI MOHAMMAD SABER, ABROOMAND AZAR PARVIZ, AHMADKHANIHA REZA, MEHDIZADEH AMIR. A RAPID, SIMPLE, LIQUID CHROMATOGRAPHIC-ELECTROSPRAY IONIZATION, ION TRAP MASS SPECTROMETRY METHOD FOR THE DETERMINATION OF FINASTERIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2012;11(1):59-67. Available from: https://sid.ir/paper/287931/en

IEEE: Copy

SYED HUSAIN HASHEMI MOUSAVI, FARZAD KOBARFARD, SYED WAQIF HUSAIN, MOHAMMAD SABER TEHRANI, PARVIZ ABROOMAND AZAR, REZA AHMADKHANIHA, and AMIR MEHDIZADEH, “A RAPID, SIMPLE, LIQUID CHROMATOGRAPHIC-ELECTROSPRAY IONIZATION, ION TRAP MASS SPECTROMETRY METHOD FOR THE DETERMINATION OF FINASTERIDE IN HUMAN PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 11, no. 1, pp. 59–67, 2012, [Online]. Available: https://sid.ir/paper/287931/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops