PREPARATION AND IN-VITRO EVALUATION OF AN ANTISENSE-CONTAINING CATIONIC LIPOSOME AGAINST NON-SMALL CELL LUNG CANCER: A COMPARATIVE PREPARATION STUDY

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SAFFARI MOSTAFA; SHIRAZI FARSHAD H.; OGHABIAN MOHAMMAD ALI; MOGHIMI HAMIDREZA

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2013 | Volume:12 | Issue:SUPPLEMENT 1 Page(s): 3-10

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Title

PREPARATION AND IN-VITRO EVALUATION OF AN ANTISENSE-CONTAINING CATIONIC LIPOSOME AGAINST NON-SMALL CELL LUNG CANCER: A COMPARATIVE PREPARATION STUDY

Author(s)

SAFFARI MOSTAFA | SHIRAZI FARSHAD H. | OGHABIAN MOHAMMAD ALI | MOGHIMI HAMIDREZA | Issue Writer Certificate

Keywords

GENE DELIVERY

LIPOSOME

THIN FILM HYDRATION

ETHANOL INJECTION

CYTOTOXICITY

Abstract

The current methods for treatment of cancers are inadequate and more specific methods suchas gene therapy are in progress. Among different vehicles, cationic LIPOSOMEs are frequently used for delivery of genetic material. This investigation aims to prepare and optimize DOTAP cationic LIPOSOMEs containing an antisense oligonuclotide (AsODN) against protein kinase C alpha in non-small cells lung cancer (NSCLC).To perform this investigation, two different methods of ETHANOL INJECTION and thin filmhydration were used to prepare AsODN-loaded DOTAP LIPOSOMEs.The formulated LIPOSOMEs were then evaluated for their morphology, particle size, zetapotential and encapsulation efficiency, and the best formulation was chosen. In-vitro growth inhibitory effect of encapsulated ODN on A549 cells were evaluated by MTT and colonogenic assay. The physical and serum stability of liposomal ODN were also evaluated.Thin film hydration method resulted in large LIPOSOMEs that required downsizing by extrusion with an encapsulation efficiency of 13%. ETHANOL INJECTION, in a single step gave LIPOSOMEs with a small size of 115 nm and an encapsulation efficiency of around 90% whichwere physically stable for 6 months. The optimized LIPOSOME could protect oligonucleotides from degradation by nuclease. Cell studies showed a 20% sequence-specific inhibition of cell growth in MTT assay and revealed an LC50 of 103 nM in colonogenic studies.In conclusion, ETHANOL INJECTION was able to provide suitable LIPOSOMEs from the permanently charged DOTAP. Also the resulted LIPOSOMEs were able to inhibit the growth of lung cancer cells.

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APA: Copy

SAFFARI, MOSTAFA, SHIRAZI, FARSHAD H., OGHABIAN, MOHAMMAD ALI, & MOGHIMI, HAMIDREZA. (2013). PREPARATION AND IN-VITRO EVALUATION OF AN ANTISENSE-CONTAINING CATIONIC LIPOSOME AGAINST NON-SMALL CELL LUNG CANCER: A COMPARATIVE PREPARATION STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(SUPPLEMENT 1), 3-10. SID. https://sid.ir/paper/288048/en

Vancouver: Copy

SAFFARI MOSTAFA, SHIRAZI FARSHAD H., OGHABIAN MOHAMMAD ALI, MOGHIMI HAMIDREZA. PREPARATION AND IN-VITRO EVALUATION OF AN ANTISENSE-CONTAINING CATIONIC LIPOSOME AGAINST NON-SMALL CELL LUNG CANCER: A COMPARATIVE PREPARATION STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(SUPPLEMENT 1):3-10. Available from: https://sid.ir/paper/288048/en

IEEE: Copy

MOSTAFA SAFFARI, FARSHAD H. SHIRAZI, MOHAMMAD ALI OGHABIAN, and HAMIDREZA MOGHIMI, “PREPARATION AND IN-VITRO EVALUATION OF AN ANTISENSE-CONTAINING CATIONIC LIPOSOME AGAINST NON-SMALL CELL LUNG CANCER: A COMPARATIVE PREPARATION STUDY,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. SUPPLEMENT 1, pp. 3–10, 2013, [Online]. Available: https://sid.ir/paper/288048/en

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