ANTI-INFLAMMATORY EFFECTS OF OXYMATRINE THROUGH INHIBITION OF NUCLEAR FACTOR–KAPPA B AND MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION IN LIPOPOLYSACCHARIDE-INDUCED BV2 MICROGLIA CELLS

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DONG XIAO QIAO; DU QUAN; YU WEN HUA; ZHANG ZU YONG; ZHU QIANG; CHE ZHI HAO; CHEN FENG; WANG HAO; CHEN JUN

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2013 | Volume:12 | Issue:1 Page(s): 165-174

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Title

ANTI-INFLAMMATORY EFFECTS OF OXYMATRINE THROUGH INHIBITION OF NUCLEAR FACTOR–KAPPA B AND MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION IN LIPOPOLYSACCHARIDE-INDUCED BV2 MICROGLIA CELLS

Author(s)

Keywords

MICROGLIA

OXYMATRINE

NUCLEAR FACTOR KAPPA-B

MITOGEN-ACTIVATED PROTEIN KINASE

INFLAMMATION

Abstract

Oxymatrine, a potent monosomic alkaloid extracted from Chinese herbSophora japonica (Sophora flavescens Ait.). possesses anti-inflammatory activittyes. This study was designed to investigate the effects of OXYMATRINE on nuclear factor–kappa B (NF- k B) and MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) -dependent inflammatory responses in lipopolysaccharide (LPS) -activated MICROGLIA. In this paper, BV2 MICROGLIA were pretreated with different concentrations of OXYMATRINE (1, 10 and 20 mg/mL) for 30 min as followed by stimulation with LPS (1 mg/mL) for different times (30 min, 1 h, 3 h, and 6 h). Concentrations of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF- a), interleukin-1beta (IL-1 b) and interleukin-6 (IL-6) in supernatant, mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), cytosolic inhibitor of kappa B-alpha (I-kB a) and phospho- I-kB a and nuclear p65 protein levels, and the phosphorylations of MAPK molecules such as extracellular-signal-regulated kinase (ERK) 1/2, p38 MAPK and c-Jun N-terminal kinase (JNK) were determined. It was shown that OXYMATRINE inhibited the productions of NO, PGE2, TNF- a, IL-1 b and IL-6, attenuated the mRNA levels of iNOS and COX-2, suppressed the phosphorylation of I-kB a in cytosol, decreased the nuclear levels of p65, and also blocked ERK, p38 and JNK pathway in LPS-stimulated BV2 MICROGLIAl cells in a dose-dependent manner. According to the results; It is suggested that OXYMATRINE may attenuate inflammatory responses of MICROGLIA and could be potentially useful in modulation of inflammatory status in the brain disorders.

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APA: Copy

DONG, XIAO QIAO, DU, QUAN, YU, WEN HUA, ZHANG, ZU YONG, ZHU, QIANG, CHE, ZHI HAO, CHEN, FENG, WANG, HAO, & CHEN, JUN. (2013). ANTI-INFLAMMATORY EFFECTS OF OXYMATRINE THROUGH INHIBITION OF NUCLEAR FACTOR–KAPPA B AND MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION IN LIPOPOLYSACCHARIDE-INDUCED BV2 MICROGLIA CELLS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(1), 165-174. SID. https://sid.ir/paper/288090/en

Vancouver: Copy

DONG XIAO QIAO, DU QUAN, YU WEN HUA, ZHANG ZU YONG, ZHU QIANG, CHE ZHI HAO, CHEN FENG, WANG HAO, CHEN JUN. ANTI-INFLAMMATORY EFFECTS OF OXYMATRINE THROUGH INHIBITION OF NUCLEAR FACTOR–KAPPA B AND MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION IN LIPOPOLYSACCHARIDE-INDUCED BV2 MICROGLIA CELLS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(1):165-174. Available from: https://sid.ir/paper/288090/en

IEEE: Copy

XIAO QIAO DONG, QUAN DU, WEN HUA YU, ZU YONG ZHANG, QIANG ZHU, ZHI HAO CHE, FENG CHEN, HAO WANG, and JUN CHEN, “ANTI-INFLAMMATORY EFFECTS OF OXYMATRINE THROUGH INHIBITION OF NUCLEAR FACTOR–KAPPA B AND MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION IN LIPOPOLYSACCHARIDE-INDUCED BV2 MICROGLIA CELLS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. 1, pp. 165–174, 2013, [Online]. Available: https://sid.ir/paper/288090/en

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