OPTIMIZATION OF BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING FUSARIUM OXYSPORUM

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KORBEKANDI HASSAN; ASHARI ZEYNAB; IRAVANI SIAVASH; ABBASI SAJJAD

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Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2013 | Volume:12 | Issue:3 Page(s): 289-298

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Title

OPTIMIZATION OF BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING FUSARIUM OXYSPORUM

Author(s)

KORBEKANDI HASSAN | ASHARI ZEYNAB | IRAVANI SIAVASH | ABBASI SAJJAD | Issue Writer Certificate

Keywords

SILVER NANOPARTICLES

FUSARIUM OXYSPORUM

OPTIMIZATION

BIOSYNTHESIS

INTRACELLULAR SYNTHESIS GABAA RECEPTOR

Abstract

SILVER NANOPARTICLES are increasingly used in various fields of biotechnology and applications in the medicine. Objectives of this study were OPTIMIZATION of production of SILVER NANOPARTICLES using biotransformations by FUSARIUM OXYSPORUM, and a further study on the location of nanoparticles synthesis in this microorganism. The reaction mixture contained the following ingredients (final concentrations): AgNO3 (1-10 mM) as the biotransformation substrate, biomass as the biocatalyst, glucose (560 mM) as the electron donor, and phosphate buffer (pH= 7, 100 mM). The samples were taken from the reaction mixtures at different times, and the absorbance (430 nm) of the colloidal suspensions of SILVER NANOPARTICLES hydrosols was read freshly (without freezing) and immediately after dilution (1:40). SEM and TEM analyses were performed on selected samples. The presence of AgNO3 (0.1 mM) in the culture as enzyme inducer, and glucose (560 mM) as electron donor had positive effects on nanoparticle production. In SEM micrographs, SILVER NANOPARTICLES were almost spherical, single (25-50 nm) or in aggregates (100 nm), attached to the surface of biomass. The reaction mixture was successfully optimized to increase the yield of SILVER NANOPARTICLES production. More details of the location of nanoparticles production by this fungus were revealed, which support the hypothesis that SILVER NANOPARTICLES are synthesized intracellularly and not extracellularly.

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APA: Copy

KORBEKANDI, HASSAN, ASHARI, ZEYNAB, IRAVANI, SIAVASH, & ABBASI, SAJJAD. (2013). OPTIMIZATION OF BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING FUSARIUM OXYSPORUM. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(3), 289-298. SID. https://sid.ir/paper/288133/en

Vancouver: Copy

KORBEKANDI HASSAN, ASHARI ZEYNAB, IRAVANI SIAVASH, ABBASI SAJJAD. OPTIMIZATION OF BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING FUSARIUM OXYSPORUM. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(3):289-298. Available from: https://sid.ir/paper/288133/en

IEEE: Copy

HASSAN KORBEKANDI, ZEYNAB ASHARI, SIAVASH IRAVANI, and SAJJAD ABBASI, “OPTIMIZATION OF BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING FUSARIUM OXYSPORUM,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. 3, pp. 289–298, 2013, [Online]. Available: https://sid.ir/paper/288133/en

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