LIQUID CHROMATOGRAPHY TANDEM MASS/MASS SPECTROMETRY FOR THE QUANTIFICATION OF FUDOSTEINE IN HUMAN SERUM WITHOUT PRECOLUMN DERIVATIZATION

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FAN PENG CHENG; REN JUN; MA HUI PING; JING LIN LIN; MA JUN; JIA ZHENG PING

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Journal: Iranian Journal of Pharmaceutical Research Year:2014 | Volume:13 | Issue:2 Page(s): 441-447

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Title

LIQUID CHROMATOGRAPHY TANDEM MASS/MASS SPECTROMETRY FOR THE QUANTIFICATION OF FUDOSTEINE IN HUMAN SERUM WITHOUT PRECOLUMN DERIVATIZATION

Author(s)

Keywords

FUDOSTEINE

HPLC-ESI/MS/MS

HUMAN SERUM

PHARMACOKINETICS

Abstract

A quantitative analysis method for FUDOSTEINE in HUMAN SERUM by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/ min. The overall chromatographic run time was approximately 7 min. The autosampler was set with an injection volume of 10 mL. The calibration curve was linear in the concentration range of 0.1~15.0 mg/mL. The coefficient of determination (r) was greater than 0.9998. This method has been fully validated and shown to be specific, accurate and precise. The method was simple, rapid and the sample preparation was minimal. It was successfully applied to the analysis of healthy volunteer

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APA: Copy

FAN, PENG CHENG, REN, JUN, MA, HUI PING, JING, LIN LIN, MA, JUN, & JIA, ZHENG PING. (2014). LIQUID CHROMATOGRAPHY TANDEM MASS/MASS SPECTROMETRY FOR THE QUANTIFICATION OF FUDOSTEINE IN HUMAN SERUM WITHOUT PRECOLUMN DERIVATIZATION. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 13(2), 441-447. SID. https://sid.ir/paper/288230/en

Vancouver: Copy

FAN PENG CHENG, REN JUN, MA HUI PING, JING LIN LIN, MA JUN, JIA ZHENG PING. LIQUID CHROMATOGRAPHY TANDEM MASS/MASS SPECTROMETRY FOR THE QUANTIFICATION OF FUDOSTEINE IN HUMAN SERUM WITHOUT PRECOLUMN DERIVATIZATION. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2014;13(2):441-447. Available from: https://sid.ir/paper/288230/en

IEEE: Copy

PENG CHENG FAN, JUN REN, HUI PING MA, LIN LIN JING, JUN MA, and ZHENG PING JIA, “LIQUID CHROMATOGRAPHY TANDEM MASS/MASS SPECTROMETRY FOR THE QUANTIFICATION OF FUDOSTEINE IN HUMAN SERUM WITHOUT PRECOLUMN DERIVATIZATION,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 13, no. 2, pp. 441–447, 2014, [Online]. Available: https://sid.ir/paper/288230/en

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