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Information Journal Paper

Title

SIMPLE, FAST AND RELIABLE LIQUID CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THEOPHYLLINE IN URINE, SALIVA AND PLASMA SAMPLES

Pages

  431-439

Abstract

 In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of THEOPHYLLINE in biological fluids.Liquid- liquid extraction is performed for isolation of the drug and elimination of PLASMA and SALIVA interferences. URINE samples were applied without any extraction. The chromatographic separation was achieved on a C18 column by using 60: 40 methanol: water as mobile phase under isocratic conditions at a flow rate of 0.75 mL/min with UV detection at 280 nm in HPLC method. UV spectrophotometric analysis was performed at 275 nm.The results of HPLC analysis were as follows: the limit of quantification: 1.1 mg/mL for URINE, 1.9 mg/mL for SALIVA, 3.1 mg/mL for PLASMA; recovery: 94.85% for PLASMA, 100.45% for SALIVA, 101.39% for URINE; intra-day precision: 0.22–2.33%, inter-day precision: 3.17-13.12%. Spectrophotometric analysis results were as follows: the limit of quantitation: 5.23 mg/mL for PLASMA, 8.7 mg/mL for URINE; recovery: 98.27% for PLASMA, 95.25% for URINE; intra-day precision: 2.37-3.00%, inter-day precision: 5.43-7.91%.It can be concluded that this validated HPLC method is easy, precise, accurate, sensitive and selective for determination of THEOPHYLLINE in biological samples. Also spectrophotometric analysis can be used where it can be applicable.

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    • Cite

    APA: Copy

    CHAREHSAZ, MOHAMMAD, GURBAY, AYLIN, AYDIN, AHMET, & SAHIN, GONUL. (2014). SIMPLE, FAST AND RELIABLE LIQUID CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THEOPHYLLINE IN URINE, SALIVA AND PLASMA SAMPLES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 13(2), 431-439. SID. https://sid.ir/paper/288249/en

    Vancouver: Copy

    CHAREHSAZ MOHAMMAD, GURBAY AYLIN, AYDIN AHMET, SAHIN GONUL. SIMPLE, FAST AND RELIABLE LIQUID CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THEOPHYLLINE IN URINE, SALIVA AND PLASMA SAMPLES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2014;13(2):431-439. Available from: https://sid.ir/paper/288249/en

    IEEE: Copy

    MOHAMMAD CHAREHSAZ, AYLIN GURBAY, AHMET AYDIN, and GONUL SAHIN, “SIMPLE, FAST AND RELIABLE LIQUID CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THEOPHYLLINE IN URINE, SALIVA AND PLASMA SAMPLES,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 13, no. 2, pp. 431–439, 2014, [Online]. Available: https://sid.ir/paper/288249/en

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