Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAMALI BARDIA; NAKHJAVANI MARYAM; HOSSEINZADEH LEILA; AMIDI SALIMEH; NIKOUNEZHAD NASTARAN; SHIRAZI FARSHAD H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Pharmaceutical Research Year:2015 | Volume:14 | Issue:2 Page(s): 513-519

Download Full-Text

View:

224

Download:

110

Cites:

Information Journal Paper

Title

Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells

Author(s)

Keywords

A375

A2780s

A2780CP

HepG2

U373MGS

U373 MGCP

GSH

Cisplatin

Abstract

One of the major complications in cancer chemotherapy with Cisplatin as one of the important medicines in treatment regimens of different cancers is the development of resistance. One of the most described cellular defense mechanisms involved in resistance is glutathione (GSH), thus in this study, the effects of Cisplatin on the total intracellular GSH level (GSHi) in some sensitive and resistant variants of human cell lines (hepatocarcinoma HepG2, sking A375, Cisplatin sensitive glioblastoma U373MG and Cisplatin resistant glioblastoma U373MGCP, Cisplatin sensitive ovary A2780s and Cisplatin resistant A2780CP cells) were studied. MTT assay was performed to measure cytotoxicity of Cisplatin (33. 3 μ M for 1 hour). Following Cisplatin exposure, GSHi (per million cells) was evaluated using a photometrical assay up to 90 minutes. Our results indicate that there are significant differences between GSHi content of A2780CP and U373MGCP cells compared to other cell lines. Moreover, IC50 of Cisplatin in different cells seems to have a relation with mean of GSH level in 90 minutes (GSH (mean)90). As a conclusion, it seems that resistance to Cisplatin in different cell lines is more related with the diverse patterns of GSHi variations following Cisplatin exposure than its original level, and/or its cellular increase or decrease. It is also suggested that GSH (mean)90 may be used as a factor for the prediction of cellular resistance to Cisplatin.

Cites

No record.

References

No record.

Cite

APA: Copy

JAMALI, BARDIA, NAKHJAVANI, MARYAM, HOSSEINZADEH, LEILA, AMIDI, SALIMEH, NIKOUNEZHAD, NASTARAN, & SHIRAZI, FARSHAD H.. (2015). Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 14(2), 513-519. SID. https://sid.ir/paper/288440/en

Vancouver: Copy

JAMALI BARDIA, NAKHJAVANI MARYAM, HOSSEINZADEH LEILA, AMIDI SALIMEH, NIKOUNEZHAD NASTARAN, SHIRAZI FARSHAD H.. Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2015;14(2):513-519. Available from: https://sid.ir/paper/288440/en

IEEE: Copy

BARDIA JAMALI, MARYAM NAKHJAVANI, LEILA HOSSEINZADEH, SALIMEH AMIDI, NASTARAN NIKOUNEZHAD, and FARSHAD H. SHIRAZI, “Intracellular GSH Alterations and Its Relationship to Level of Resistance following Exposure to Cisplatin in Cancer Cells,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 14, no. 2, pp. 513–519, 2015, [Online]. Available: https://sid.ir/paper/288440/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops