Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles

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Fadaeian Ghazal; SHOJAOSADATI SEYED ABBAS; Kouchakzadeh Hasan; SHOKRI FAZEL; SOLEIMANI MASOUD

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2015 | Volume:14 | Issue:2 Page(s): 395-405

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Title

Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles

Author(s)

Keywords

5-Flourouracil

Bovine serum albumin nanoparticles

1F2 monoclonal antibody

Abstract

Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23 ± 4%) obtained in comparison to N-succinimidyl 3-(2-Pyridyl Dithio) Propionate (SPDP) (8 ± 2%). A slight increase in the average particle size with a negligible prolongation of the 5-FU release was observed after 1F2 coupling. The 1F2-coupled 5-FU-loaded BSA nanoparticles interacted with nearly all HER2 receptors available on the surface of HER2-positive SKBR3 cells. No cellular uptake was observed for HER2-negative MCF7 cells. Physicochemical and biological properties of the mAb-modified nanoparticles did not significantly alter after three months of storage at room temperature. The in-vitro cytotoxicity evaluation by MTT assay, demonstrated lower SKBR3 viability (50. 7 ± 9 %) after 5 hours contact with 1F2-coupled 5-FU-loaded BSA nanoparticles in comparison with the other control systems due to their cell attachment and internalization after washing. In addition, no significant toxicity was observed on MCF7 cells. This novel system can efficiently be employed for targeted delivery of 5-FU to HER2-positive cancerous cells.

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APA: Copy

Fadaeian, Ghazal, SHOJAOSADATI, SEYED ABBAS, Kouchakzadeh, Hasan, SHOKRI, FAZEL, & SOLEIMANI, MASOUD. (2015). Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 14(2), 395-405. SID. https://sid.ir/paper/288460/en

Vancouver: Copy

Fadaeian Ghazal, SHOJAOSADATI SEYED ABBAS, Kouchakzadeh Hasan, SHOKRI FAZEL, SOLEIMANI MASOUD. Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2015;14(2):395-405. Available from: https://sid.ir/paper/288460/en

IEEE: Copy

Ghazal Fadaeian, SEYED ABBAS SHOJAOSADATI, Hasan Kouchakzadeh, FAZEL SHOKRI, and MASOUD SOLEIMANI, “Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 14, no. 2, pp. 395–405, 2015, [Online]. Available: https://sid.ir/paper/288460/en

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