Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques

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PAKRAVAN PARVANEH; MASOUDIAN SHAHLA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian Journal of Pharmaceutical Research Year:2015 | Volume:14 | Issue:1 Page(s): 111-123

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Title

Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques

Author(s)

PAKRAVAN PARVANEH | MASOUDIAN SHAHLA | Issue Writer Certificate

Keywords

CT-DNA

Isatin-β

-thiosemicarbazone

Neutral Red dye

Intercalation

Abstract

The interaction between Isatin-β;-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7. 4) using Neutral Red (NR) dye as a spectral probe by UV– Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by Intercalation in the DNA (K [IBT – DNA] = 1. 03×105 M− 1), and displaces the NR dye from the NR– DNA complex. The binding constants Kf and number of binding sites (n≈ 1) of IBT with DNA were obtained by fluorescence quenching method at different temperatures. Furthermore, the enthalpy and entropy of the reaction between IBT and CT-DNA showed that the reaction is enthalpy-favored and entropy-disfavored. The changes in the base stacking of CT-DNA upon the binding of IBT are reflected in the circular dichroic (CD) spectral studies. The viscosity increase of CT-DNA solution is another evidence to indicate that, IBT is able to be intercalated in the DNA base pairs.

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APA: Copy

PAKRAVAN, PARVANEH, & MASOUDIAN, SHAHLA. (2015). Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 14(1), 111-123. SID. https://sid.ir/paper/288493/en

Vancouver: Copy

PAKRAVAN PARVANEH, MASOUDIAN SHAHLA. Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2015;14(1):111-123. Available from: https://sid.ir/paper/288493/en

IEEE: Copy

PARVANEH PAKRAVAN, and SHAHLA MASOUDIAN, “Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 14, no. 1, pp. 111–123, 2015, [Online]. Available: https://sid.ir/paper/288493/en

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