Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2. 0 Cells

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JAHANDAR HODA; VAZIRI BEHROUZ; NEMATOLLAHI LEILA; Afsharirad Tayebeh; MIRABZADEH ESMAT; TORKASHVAND FATEMEH; KHALAJ VAHID

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Journal: Iranian Journal of Pharmaceutical Research Year:2015 | Volume:14 | Issue:1 Page(s): 177-187

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Title

Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2. 0 Cells

Author(s)

Keywords

Cysteamine

IgG4

Monoclonal antibody

Protein disulfide isomerase

Sp2.0 cells

Thiol reducing agents

Abstract

The manipulation of redox potential in secretory pathway by Thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. Here we have studied the influence of Cysteamine on viability and the production level of IgG4 in Sp2. 0 cells. For this purpose, the recombinant Sp2. 0 cells producing an anti CD33 IgG4, were subjected to different concentrations of Cysteamine. At concentrations of 2, 4 and 5 mM Cysteamine, the secreted levels of IgG4 did not change significantly. However, in concentration of 7 mM Cysteamine, a significant decrease was observed in IgG4 levels which may indicate the cytotoxicity of this compound in higher concentrations. Our results show that the Cysteamine treatment reduces the cell viability in a dose-dependent manner. Also it was observed that 2 mM Cysteamine had no late effect on IgG4 production level and only at day 3, this concentration of Cysteamine decreased the cell viability significantly. To test whether the addition of Cysteamine can affect the expression level of Protein disulfide isomerase, RTPCR analysis was carried out. The results revealed that Cysteamine does not affect the PDI transcription and expression level of IgG4 in this type of recombinant cells.

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APA: Copy

JAHANDAR, HODA, VAZIRI, BEHROUZ, NEMATOLLAHI, LEILA, Afsharirad, Tayebeh, MIRABZADEH, ESMAT, TORKASHVAND, FATEMEH, & KHALAJ, VAHID. (2015). Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2. 0 Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 14(1), 177-187. SID. https://sid.ir/paper/288514/en

Vancouver: Copy

JAHANDAR HODA, VAZIRI BEHROUZ, NEMATOLLAHI LEILA, Afsharirad Tayebeh, MIRABZADEH ESMAT, TORKASHVAND FATEMEH, KHALAJ VAHID. Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2. 0 Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2015;14(1):177-187. Available from: https://sid.ir/paper/288514/en

IEEE: Copy

HODA JAHANDAR, BEHROUZ VAZIRI, LEILA NEMATOLLAHI, Tayebeh Afsharirad, ESMAT MIRABZADEH, FATEMEH TORKASHVAND, and VAHID KHALAJ, “Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2. 0 Cells,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 14, no. 1, pp. 177–187, 2015, [Online]. Available: https://sid.ir/paper/288514/en

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